Gordonia phage DoobyDoo
DoobyDoo Plaque Picture
DoobyDoo EM Picture
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Detailed Information for Phage DoobyDoo
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByAutumn Grantz
Year Found2023
Location FoundNew Albany, IN United States of America
Finding InstitutionIndiana University-Southeast
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature26°C
GPS Coordinates38.35806 N, 85.8027 W Map
Discovery NotesThe soil sample was collected from a flower garden. The soil was dark brown and moist and dug about 2 in. from the ground. Soil was collected on 9/11/2023.
Naming NotesIbrahim Abdulrehman and Preston Penny isolated DoobyDoo. We were inspired by the American animated cartoon series Scooby-Doo, with its central theme of investigation, and named our phage after the main protagonist Scoobert “Scooby” Doo. Much like on the show, the Mystery Incorporated gang is always asking, "Scooby Doo, where are you?" There was a struggle to isolate a phage sample successfully. It had us asking, "Phage sample, where are you?" Thus, the name DoobyDoo was created!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 2, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage773
Genome length (bp)66343
Character of genome endsCircularly Permuted
GC Content58.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDV
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionIndiana University-Southeast
Annotation StatusIn GenBank
Plaque NotesOur phage creates approximately 1 millimeter-in diameter plaques. The plaques appear distinctly cleared on agar plates and have defined edges. They have an irregular circular shape.
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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DoobyDoo_1

DoobyDoo_2
(terminase)

DoobyDoo_3
(portal protein)

DoobyDoo_4
(esterase)

DoobyDoo_5

DoobyDoo_6

DoobyDoo_7

DoobyDoo_8

DoobyDoo_9

DoobyDoo_10
(metalloprotease)

DoobyDoo_11
(minor capsid protein)

DoobyDoo_12
(scaffolding protein)

DoobyDoo_13
(minor capsid protein)

DoobyDoo_14
(major capsid protein)

DoobyDoo_15

DoobyDoo_16

DoobyDoo_17

DoobyDoo_18
(head-to-tail stopper)

DoobyDoo_19

DoobyDoo_20
(tail terminator)

DoobyDoo_21

DoobyDoo_22

DoobyDoo_23
(major tail protein)

DoobyDoo_24

DoobyDoo_25

DoobyDoo_26
(tape measure protein)

DoobyDoo_27
(minor tail protein)

DoobyDoo_28
(minor tail protein)

DoobyDoo_29
(minor tail protein)

DoobyDoo_30

DoobyDoo_31

DoobyDoo_32

DoobyDoo_33

DoobyDoo_34

DoobyDoo_35
(lysin A)

DoobyDoo_36

DoobyDoo_37

DoobyDoo_38

DoobyDoo_39

DoobyDoo_40

DoobyDoo_41

DoobyDoo_42

DoobyDoo_43

DoobyDoo_44

DoobyDoo_45

DoobyDoo_46

DoobyDoo_47

DoobyDoo_48

DoobyDoo_49

DoobyDoo_50

DoobyDoo_51

DoobyDoo_52

DoobyDoo_53

DoobyDoo_54

DoobyDoo_55

DoobyDoo_56

DoobyDoo_57

DoobyDoo_58

DoobyDoo_59
(DNA helicase)

DoobyDoo_60

DoobyDoo_61

DoobyDoo_62

DoobyDoo_63

DoobyDoo_64
(Cas4 exonuclease)

DoobyDoo_65

DoobyDoo_66
(ASCE ATPase)

DoobyDoo_67

DoobyDoo_68
(oxidoreductase)

DoobyDoo_69
(hydrolase)

DoobyDoo_70

DoobyDoo_71

DoobyDoo_72
(methyltransferase)

DoobyDoo_73

DoobyDoo_74
(oxidoreductase)

DoobyDoo_75

DoobyDoo_76

DoobyDoo_77

DoobyDoo_78
(DnaE-like DNA polymerase III alpha)

DoobyDoo_79
(MazG-like nucleotide pyrophosphohydrolase)

DoobyDoo_80

DoobyDoo_81

DoobyDoo_82
(ThyX-like thymidylate synthase)

DoobyDoo_83

DoobyDoo_84

DoobyDoo_85
(RuvC-like resolvase)

DoobyDoo_86
(lysin B)

DoobyDoo_87

DoobyDoo_88
(DNA primase/helicase)

DoobyDoo_89

DoobyDoo_90

DoobyDoo_91

DoobyDoo_92

DoobyDoo_93

DoobyDoo_94

DoobyDoo_95

DoobyDoo_96

DoobyDoo_97

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ362665
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 184
Pitt Freezer Box Grid# A2
Available Files
GenBank File for PhameratorDownload