| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Autumn Grantz |
| Year Found | 2023 |
| Location Found | New Albany, IN United States of America |
| Finding Institution | Indiana University-Southeast |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 26°C |
| GPS Coordinates | 38.35806 N, 85.8027 W Map |
| Discovery Notes | The soil sample was collected from a flower garden. The soil was dark brown and moist and dug about 2 in. from the ground. Soil was collected on 9/11/2023. |
| Naming Notes | Ibrahim Abdulrehman and Preston Penny isolated DoobyDoo. We were inspired by the American animated cartoon series Scooby-Doo, with its central theme of investigation, and named our phage after the main protagonist Scoobert “Scooby” Doo. Much like on the show, the Mystery Incorporated gang is always asking, "Scooby Doo, where are you?" There was a struggle to isolate a phage sample successfully. It had us asking, "Phage sample, where are you?" Thus, the name DoobyDoo was created! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 773 |
| Genome length (bp) | 66343 |
| Character of genome ends | Circularly Permuted |
| GC Content | 58.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DV |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Indiana University-Southeast |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage creates approximately 1 millimeter-in diameter plaques. The plaques appear distinctly cleared on agar plates and have defined edges. They have an irregular circular shape. |
| Number of Genes | 97 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ362665 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
184 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| GenBank File for Phamerator | Download |
