| Detailed Information for Phage DrGrey |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Meredith Shaffer |
| Year Found | 2016 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.212584 N, 97.14542 W Map |
| Discovery Notes | I gathered the dirt from near the RTFP building on the UNT campus. The dirt was moist and cool with plant particles in it when collected. It was collected at 4:10pm at 18 degrees Celsius. |
| Naming Notes | I named this phage after my favorite character in the show Grey's Anatomy. As well, Dr. Grey is one of my nicknames because I am obsessed with the show and I want to become a surgeon when I am older. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 12, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 4377 |
| Genome length (bp) | 56076 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 59.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are circular, clear, and measure to be about 1.6mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF467948 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8x10^10 pfu/mL |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
