Mycobacterium phage FreakyGoo
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Detailed Information for Phage FreakyGoo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGracie Gonzalez, Abbie Smith
Year Found2024
Location FoundBlacksburg, VA United States
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.247028 N, 80.419889 W Map
Discovery NotesDiscovered from an enriched isolation of peat soil, found in moist conditions due to rain the previous evening.
Naming NotesNamed due to a joke about all the miscellaneous liquids we worked with in order to isolate the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2025
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina NextSeq 1000
Read TypeSingle-end reads
Read Length100 bp
Approximate Shotgun Coverage2665
Genome length (bp)81298
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceACCTCCTGCAA
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterM
SubclusterM1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusQC Complete
Plaque NotesBig round plaques.
MorphotypeSiphoviridae
Number of Genes139
Number of tRNAs19
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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FreakyGoo_1

FreakyGoo_2

FreakyGoo_3

FreakyGoo_4

FreakyGoo_5

FreakyGoo_6

FreakyGoo_7

FreakyGoo_8

FreakyGoo_9

FreakyGoo_10

FreakyGoo_11

FreakyGoo_12

FreakyGoo_13

FreakyGoo_14
(terminase, small subunit)

FreakyGoo_15
(terminase, large subunit)

FreakyGoo_16
(portal protein)

FreakyGoo_17
(capsid maturation protease)

FreakyGoo_18
(scaffolding protein)

FreakyGoo_19
(major capsid protein)

FreakyGoo_20
(head-to-tail adaptor)

FreakyGoo_21
(head-to-tail stopper)

FreakyGoo_22

FreakyGoo_23
(tail terminator)

FreakyGoo_24
(major tail protein)

FreakyGoo_25
(tail assembly chaperone)

FreakyGoo_26
(tail assembly chaperone)

FreakyGoo_27
(tape measure protein)

FreakyGoo_28
(minor tail protein)

FreakyGoo_29
(minor tail protein)

FreakyGoo_30
(minor tail protein)

FreakyGoo_31
(minor tail protein)

FreakyGoo_32
(minor tail protein)

FreakyGoo_33

FreakyGoo_34

FreakyGoo_35
(minor tail protein)

FreakyGoo_36
(lysin A)

FreakyGoo_37

FreakyGoo_38
(holin)

FreakyGoo_39
(lysin B)

FreakyGoo_40
(NrdH-like glutaredoxin)

FreakyGoo_41

FreakyGoo_42

FreakyGoo_43

FreakyGoo_44

FreakyGoo_45

FreakyGoo_46

FreakyGoo_47

FreakyGoo_48
(nucleotidyl transferase)

FreakyGoo_49

FreakyGoo_50

FreakyGoo_51

FreakyGoo_52

FreakyGoo_53

FreakyGoo_54

FreakyGoo_55
(ERF family DNA pairing protein)

FreakyGoo_56

FreakyGoo_57
(exonuclease)

FreakyGoo_58

FreakyGoo_59
(ClpP-like protease)

FreakyGoo_60

FreakyGoo_61

FreakyGoo_62

FreakyGoo_63

FreakyGoo_64

FreakyGoo_65

FreakyGoo_66

FreakyGoo_67
(HNH endonuclease)

FreakyGoo_68
(SSB protein)

FreakyGoo_69

FreakyGoo_70
(HNH endonuclease)

FreakyGoo_71
(DnaB-like dsDNA helicase)

FreakyGoo_72
(WhiB family transcription factor)

FreakyGoo_73
(Holliday junction resolvase)

FreakyGoo_74
(WhiB family transcription factor)

FreakyGoo_75

FreakyGoo_76

FreakyGoo_77

FreakyGoo_78

FreakyGoo_79
(endonuclease VII)

FreakyGoo_80

FreakyGoo_81
(DnaQ-like (DNA polymerase III subunit))

FreakyGoo_82

FreakyGoo_83

FreakyGoo_84
(HNH endonuclease)

FreakyGoo_85
(PTPc tyrosine phosphatase)

FreakyGoo_86

FreakyGoo_87

FreakyGoo_88

FreakyGoo_89

FreakyGoo_90

FreakyGoo_91
(HNH endonuclease)

FreakyGoo_92

FreakyGoo_93 — tRNA
(tRNA-Trp(cca))

FreakyGoo_94 — tRNA
(tRNA-Asn(gtt))

FreakyGoo_95

FreakyGoo_96 — tRNA
(tRNA-Arg(acg))

FreakyGoo_97 — tRNA
(tRNA-Tyr(gta))

FreakyGoo_98 — tRNA
(tRNA-Gln(ctg))

FreakyGoo_99 — tRNA
(tRNA-Pro(tgg))

FreakyGoo_100 — tRNA
(tRNA-Ser(gct))

FreakyGoo_101 — tRNA
(tRNA-Phe(gaa))

FreakyGoo_102 — tRNA
(tRNA-Met(cat))

FreakyGoo_103

FreakyGoo_104 — tRNA
(tRNA-Arg(acg))

FreakyGoo_105 — tRNA
(tRNA-His(gtg))

FreakyGoo_106 — tRNA
(tRNA-Cys(gca))

FreakyGoo_107

FreakyGoo_108 — tRNA
(tRNA-Leu(cag))

FreakyGoo_109
(ribonucleotide reductase)

FreakyGoo_110 — tRNA
(tRNA-Lys(ctt))

FreakyGoo_111 — tRNA
(tRNA-Gly(tcc))

FreakyGoo_112 — tRNA
(tRNA-Val(tac))

FreakyGoo_113 — tRNA
(tRNA-Thr(agt))

FreakyGoo_114 — tRNA
(tRNA-Asp(gtc))

FreakyGoo_115 — tRNA
(tRNA-Glu(ctc))

FreakyGoo_116
(rtcB-like RNA ligase)

FreakyGoo_117
(membrane protein, Band-7-like)

FreakyGoo_118
(MazG-like nucleotide pyrophosphohydrolase)

FreakyGoo_119

FreakyGoo_120

FreakyGoo_121

FreakyGoo_122

FreakyGoo_123

FreakyGoo_124

FreakyGoo_125
(DprA-like DNA processing chain A)

FreakyGoo_126
(phosphoesterase)

FreakyGoo_127

FreakyGoo_128

FreakyGoo_129

FreakyGoo_130

FreakyGoo_131
(serine integrase)

FreakyGoo_132

FreakyGoo_133

FreakyGoo_134

FreakyGoo_135

FreakyGoo_136

FreakyGoo_137

FreakyGoo_138

FreakyGoo_139

FreakyGoo_140

FreakyGoo_141

FreakyGoo_142

FreakyGoo_143

FreakyGoo_144

FreakyGoo_145

FreakyGoo_146

FreakyGoo_147

FreakyGoo_148

FreakyGoo_149

FreakyGoo_150

FreakyGoo_151

FreakyGoo_152

FreakyGoo_153

FreakyGoo_154

FreakyGoo_155

FreakyGoo_156

FreakyGoo_157

FreakyGoo_158

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 210
Pitt Freezer Box Grid# H2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload