| Detailed Information for Phage FreakyGoo |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gracie Gonzalez, Abbie Smith |
| Year Found | 2024 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.247028 N, 80.419889 W Map |
| Discovery Notes | Discovered from an enriched isolation of peat soil, found in moist conditions due to rain the previous evening. |
| Naming Notes | Named due to a joke about all the miscellaneous liquids we worked with in order to isolate the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2665 |
| Genome length (bp) | 81298 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | Big round plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 139 |
| Number of tRNAs | 19 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915829 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
210 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
