| Detailed Information for Phage Gomashi |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Blossom |
| Found By | Daphne Hussey |
| Year Found | 2012 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.851325 N, 92.614663 W Map |
| Discovery Notes | I found my soil outside of Ames Suites on the UWRF campus. The area where I found it was low traffic and in the shade. I dug about 4-5 inches into the dirt where it was cool and moist. |
| Naming Notes | My phage was super small so I named it Gomashi, which is a Japanese word meaning "little sesame seed". I felt this fit my phage because of their size. Its also the name of my cat. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 7, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 360 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 41903 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | When we first started this process my plaque were a little larger and had halos around them. However, after we isolated them I found that they were really small, and there were a lot of them. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM923970 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4x10^11 |
| Date of SEA Lysate Titering |
Oct 15, 2012 |
| Pitt Freezer Box# |
41 |
| Pitt Freezer Box Grid# |
40 |