| Detailed Information for Phage Hydrus | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Jackson Bland, Sydney Miller |
| Year Found | 2020 |
| Location Found | Tampa, FL USA |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 29.511 N, 82.231 W Map |
| Discovery Notes | Tampa Palms 5, collected in compost area near hole #14 under foliage waste; collected at 82F. Soil wet after rain. |
| Naming Notes | The Hydrus constellation essentially means water snake, a type of snake that is nonvenomous to us and swallows its prey alive. Some species are more aggressive than others. We named it Hydrus due to the aquatic aspect, and there was lots of rain prior to the collection of the phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 664 |
| Genome length (bp) | 60842 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic, infected both Gordonia Rubripertincta (host) and Gordonia Terrae. |
| Other Cluster Members | |
| Annotating Institution | University of South Florida |
| Annotation Status | Being Annotated (Expected completion by 5/1/2023) |
| Plaque Notes | Plaques were lytic, small/medium sized, mostly clear, some bullseye. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHydrus_Draft_1 Hydrus_Draft_2 Hydrus_Draft_3 Hydrus_Draft_4 Hydrus_Draft_5 Hydrus_Draft_6 Hydrus_Draft_7 Hydrus_Draft_8 Hydrus_Draft_9 Hydrus_Draft_10 Hydrus_Draft_11 Hydrus_Draft_12 Hydrus_Draft_13 Hydrus_Draft_14 Hydrus_Draft_15 Hydrus_Draft_16 Hydrus_Draft_17 Hydrus_Draft_18 Hydrus_Draft_19 Hydrus_Draft_20 Hydrus_Draft_21 Hydrus_Draft_22 Hydrus_Draft_23 Hydrus_Draft_24 Hydrus_Draft_25 Hydrus_Draft_26 Hydrus_Draft_27 Hydrus_Draft_28 Hydrus_Draft_29 Hydrus_Draft_30 Hydrus_Draft_31 Hydrus_Draft_32 Hydrus_Draft_33 Hydrus_Draft_34 Hydrus_Draft_35 Hydrus_Draft_36 Hydrus_Draft_37 Hydrus_Draft_38 Hydrus_Draft_39 Hydrus_Draft_40 Hydrus_Draft_41 Hydrus_Draft_42 Hydrus_Draft_43 Hydrus_Draft_44 Hydrus_Draft_45 Hydrus_Draft_46 Hydrus_Draft_47 Hydrus_Draft_48 Hydrus_Draft_49 Hydrus_Draft_50 Hydrus_Draft_51 Hydrus_Draft_52 Hydrus_Draft_53 Hydrus_Draft_54 Hydrus_Draft_55 Hydrus_Draft_56 Hydrus_Draft_57 Hydrus_Draft_58 Hydrus_Draft_59 Hydrus_Draft_60 Hydrus_Draft_61 Hydrus_Draft_62 Hydrus_Draft_63 Hydrus_Draft_64 Hydrus_Draft_65 Hydrus_Draft_66 Hydrus_Draft_67 Hydrus_Draft_68 Hydrus_Draft_69 Hydrus_Draft_70 Hydrus_Draft_71 Hydrus_Draft_72 Hydrus_Draft_73 Hydrus_Draft_74 Hydrus_Draft_75 Hydrus_Draft_76 Hydrus_Draft_77 Hydrus_Draft_78 Hydrus_Draft_79 Hydrus_Draft_80 Hydrus_Draft_81 Hydrus_Draft_82 Hydrus_Draft_83 Hydrus_Draft_84 Hydrus_Draft_85 Hydrus_Draft_86 Hydrus_Draft_87 Hydrus_Draft_88 Hydrus_Draft_89 Hydrus_Draft_90 Hydrus_Draft_91 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.07 x 10^10 PFU/ml |
| Pitt Freezer Box# | 118 |
| Pitt Freezer Box Grid# | E7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
