Gordonia phage Hydrus
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Detailed Information for Phage Hydrus
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByJackson Bland, Sydney Miller
Year Found2020
Location FoundTampa, FL USA
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates29.511 N, 82.231 W Map
Discovery NotesTampa Palms 5, collected in compost area near hole #14 under foliage waste; collected at 82F. Soil wet after rain.
Naming NotesThe Hydrus constellation essentially means water snake, a type of snake that is nonvenomous to us and swallows its prey alive. Some species are more aggressive than others. We named it Hydrus due to the aquatic aspect, and there was lots of rain prior to the collection of the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage664
Genome length (bp)60842
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCTCT
GC Content51.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDJ
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesLytic, infected both Gordonia Rubripertincta (host) and Gordonia Terrae.
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesPlaques were lytic, small/medium sized, mostly clear, some bullseye.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.07 x 10^10 PFU/ml
Pitt Freezer Box# 118
Pitt Freezer Box Grid# E7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload