Gordonia phage Nithya
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Detailed Information for Phage Nithya
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByXavier Rodriguez and Haley Sawyer
Year Found2020
Location FoundLincoln, NE United States of America
Finding InstitutionUniversity of Nebraska-Lincoln
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature32°C
GPS Coordinates40.8195 N, 96.6845 W Map
Discovery NotesThe soil sample came from a community garden. The depth of soil was around 2cm and the soil was also dry and dusty. The garden was jointly Nithya’s and Ellie’s.
Naming NotesFound by Xavier.

Xavier: I called my friend last minute asking if I could take her soil, so it's only fair that I name the phage after her. I would like to thank Ellie for letting me come to her garden on such short notice. I also want to thank Haley for being such a great lab partner this semester (F2020) and Dr. Chia for teaching this course.

Pronounced: Nih-thyah like “with ya”.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage374
Genome length (bp)61092
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCTCT
GC Content51.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDJ
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesBecause the plaques are so small, it is hard to tell whether Nithya is lytic or lysogenic. We believe that Nithya is lytic because the plaques tend to be clear and not turbid.
Other Cluster Members
Annotating InstitutionUniversity of Nebraska-Lincoln
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesThe plaques are very small (less than 0.5mm on average). The plaques do not appear to be turbid and they have very clear edges.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 121
Pitt Freezer Box Grid# A7
Available Files
Plaque PictureDownload
EM PictureDownload