Gordonia phage Figliar
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Detailed Information for Phage Figliar
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByAnna Harshaw
Year Found2020
Location FoundLas Vegas, NV United States
Finding InstitutionCollege of Southern Nevada
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature38°C
GPS Coordinates36.155333 N, 115.265056 W Map
Discovery NotesSample picked in a dog park. Fecal matter was present in the area. Relatively moist area due to system of sprinklers.
Naming Notes"Figliar" is an old-fashioned Russian word. It translates as "trickster" or "someone hard to deal with." Due to the lysogenic nature of found bacteriophage, most of the procedures required additional steps to complete. In general, it's complicated to work with.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage793
Genome length (bp)61147
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCTCT
GC Content51.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDJ
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesAt first, was thought as a lysogenic phage. Lately revealed to be lytic. Plaques are small in diameter and cloudy.
Other Cluster Members
Annotating InstitutionCollege of Southern Nevada
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesPlaques are small in diameter (1mm-2mm), cloudy and hard to see.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.8*10^9pfu/ml
Pitt Freezer Box# 117
Pitt Freezer Box Grid# E1
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload