Gordonia phage Secretariat
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Detailed Information for Phage Secretariat
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Found ByMae Horne
Year Found2019
Location FoundLutz, FL United States
Finding InstitutionUniversity of South Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature33°C
GPS Coordinates28.94 N, 82.42 W Map
Discovery NotesSoil Sample taken from underneath a pile of horse manure in an area of high biodiversity in plants and fungi. Soil was in a hot, dry, and dark location.
Naming NotesI named the phage after the triple crown winner, Secretariat. He is known as one of the greatest racing horses in history due to his above average speed. My phage reproduced at a fast rate and had an extremely high titer. I also found the phage under a pile of horse manure at the local horse stables.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 30, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage860
Genome length (bp)57731
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCCGCTCT
GC Content52.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDJ
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesPhage Secretariat underwent the Lytic Reproductive cycle, as was determined by the clear plaques.
Other Cluster Members
Annotating InstitutionUniversity of South Florida
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesThe plaques observed in a uniform population were of an average size, not small and not large. The plaques were clear, round, and lytic.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload