| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Mae Horne |
| Year Found | 2019 |
| Location Found | Lutz, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 33°C |
| GPS Coordinates | 28.94 N, 82.42 W Map |
| Discovery Notes | Soil Sample taken from underneath a pile of horse manure in an area of high biodiversity in plants and fungi. Soil was in a hot, dry, and dark location. |
| Naming Notes | I named the phage after the triple crown winner, Secretariat. He is known as one of the greatest racing horses in history due to his above average speed. My phage reproduced at a fast rate and had an extremely high titer. I also found the phage under a pile of horse manure at the local horse stables. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 860 |
| Genome length (bp) | 57731 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 52.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage Secretariat underwent the Lytic Reproductive cycle, as was determined by the clear plaques. |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques observed in a uniform population were of an average size, not small and not large. The plaques were clear, round, and lytic. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310850 |
| Refseq Number | None yet |
| Published in Paper | Bland et al 2023, Microbiology Resource Announcements: Annotation of Secretariat and Hydrus, two DJ cluster phages isolated on Gordonia rubripertincta |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
97 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
