| Detailed Information for Phage Secretariat | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Mae Horne |
| Year Found | 2019 |
| Location Found | Lutz, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 33°C |
| GPS Coordinates | 28.94 N, 82.42 W Map |
| Discovery Notes | Soil Sample taken from underneath a pile of horse manure in an area of high biodiversity in plants and fungi. Soil was in a hot, dry, and dark location. |
| Naming Notes | I named the phage after the triple crown winner, Secretariat. He is known as one of the greatest racing horses in history due to his above average speed. My phage reproduced at a fast rate and had an extremely high titer. I also found the phage under a pile of horse manure at the local horse stables. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 860 |
| Genome length (bp) | 57731 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 52.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage Secretariat underwent the Lytic Reproductive cycle, as was determined by the clear plaques. |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques observed in a uniform population were of an average size, not small and not large. The plaques were clear, round, and lytic. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSecretariat_1 Secretariat_2 Secretariat_3 Secretariat_4 Secretariat_5 Secretariat_6 Secretariat_7 Secretariat_8 Secretariat_9 Secretariat_10 Secretariat_11 Secretariat_12 Secretariat_13 Secretariat_14 Secretariat_15 Secretariat_16 Secretariat_17 Secretariat_18 Secretariat_19 Secretariat_20 Secretariat_21 Secretariat_22 Secretariat_23 Secretariat_24 Secretariat_25 Secretariat_26 Secretariat_27 Secretariat_28 Secretariat_29 Secretariat_30 Secretariat_31 Secretariat_32 Secretariat_33 Secretariat_34 Secretariat_35 Secretariat_36 Secretariat_37 Secretariat_38 Secretariat_39 Secretariat_40 Secretariat_41 Secretariat_42 Secretariat_43 Secretariat_44 Secretariat_45 Secretariat_46 Secretariat_47 Secretariat_48 Secretariat_49 Secretariat_50 Secretariat_51 Secretariat_52 Secretariat_53 Secretariat_54 Secretariat_55 Secretariat_56 Secretariat_57 Secretariat_58 Secretariat_59 Secretariat_60 Secretariat_61 Secretariat_62 Secretariat_63 Secretariat_64 Secretariat_65 Secretariat_66 Secretariat_67 Secretariat_68 Secretariat_69 Secretariat_70 Secretariat_71 Secretariat_72 Secretariat_73 Secretariat_74 Secretariat_75 Secretariat_76 Secretariat_77 Secretariat_78 Secretariat_79 Secretariat_80 Secretariat_81 Secretariat_82 Secretariat_83 Secretariat_84 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT310850 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 97 |
| Pitt Freezer Box Grid# | E12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
