| Detailed Information for Phage Jolene |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Asli Ozdemir |
| Year Found | 2020 |
| Location Found | Las Vegas, NV United States of America |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 36.109722 N, 115.1425 W Map |
| Discovery Notes | The sample was collected from a sample of Miracle Grow Moisture Control Potting Mix. The soil was dry, granular, dark brown, and sediment rich. It was collected at 36° 6' 35" N, 115° 8' 33" W at an elevation of 612 meters with a 5% humidity index and a temperature of 21°C. |
| Naming Notes | Named after my sister's favorite Dolly Parton song. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 156 |
| Genome length (bp) | 42305 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques ranged from 2 mm to 2.75 mm in diameter. They were all symmetrical, round, clear, and smooth. All plaques were consistent in shape and size. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ171377 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
120 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| GenBank File for Phamerator | Download |