| Detailed Information for Phage LesNorah |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Veronica Alvarado Fernandez |
| Year Found | 2021 |
| Location Found | Tampa, FL United States |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 29°C |
| GPS Coordinates | 28.061616 N, 82.418989 W Map |
| Discovery Notes | The soil sample was collected 10 minutes after a continuous day of raining adjacent to a man-made lake. The soil consistency was a little dry and the sample was surrounded by grass. |
| Naming Notes | I chose to name my phage LesNorah after my dad, Leslie, and my mom, Norah. They have been my biggest fans in everything I do. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 29, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 229 |
| Genome length (bp) | 54521 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EK |
| Subcluster | EK1 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques are medium to large, clear, and lytic plaques. My phage completely lyses the bacteria present in every infection. |
| Morphotype | Podoviridae |
| Number of Genes | 54 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OM913587 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.56 x 10^9 |
| Pitt Freezer Box# |
132 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
