| Detailed Information for Phage Marmie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meliya Hart |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 3°C |
| GPS Coordinates | 39.946662 N, 75.204501 W Map |
| Discovery Notes | Soil was moist and was collected from 3 centimeters deep. It was found next to (about five feet away) an old storage building in the cemetery. Snow melted in the area the sample was taken, but the surrounding area had about a quarter inch of snow. Ground is slightly sloped and the area sample was taken from was closer to the top of the slope. There is minimal foot traffic because it is off of the paved walking path. There are several trees around, the closest being about ten feet away. There was a second house about 200 feet away and a garden area about 50 feet away. |
| Naming Notes | Named after my dog Marmie since my two other dogs' names, Samson and Primrose, were taken. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Oct 28, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1472 |
| Genome length (bp) | 41902 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque size ranged from between 1/2 millimeter to 1 millimeter. Plaques are clear and round making this Phage a lytic phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON637766 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3x10^9 |
| Pitt Freezer Box# |
130 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
