| Detailed Information for Phage PinkYoshi |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Noelle Nocon |
| Year Found | 2021 |
| Location Found | Blacksburg, VA United States |
| Finding Institution | Virginia Tech |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.253312 N, 80.415113 W Map |
| Discovery Notes | -Suburban neighborhood
-Cul-de-sac of non-busy street
-Soil of tree on the perimeter of the street
-Next to sidewalk with potential foot traffic of people and animals (dogs/cats) |
| Naming Notes | Chose this name because pink Yoshi is who I main in the game Super Smash Bros! Yes––Yoshi has to be pink for me to win. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 3, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3131 |
| Genome length (bp) | 41893 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Tech |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, some plaques bigger than others but a majority are bigger |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON392162 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
134 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
