| Detailed Information for Phage Pirate | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Yu Tang Mei |
| Year Found | 2013 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.4 N, 118.27 W Map |
| Discovery Notes | Isolated from nasal microcomedones. Phage isolation and genome annotation performed by Eleanor Chang, Laurel Clare, Yu Tang Mei, Cinay Murguia, and Kevin Tran (UCLA). DNA preparation prepared by Krisanavane Reddi (UCLA). Assembly performed by Will Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA) |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 24, 2014 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 246 |
| Genome length (bp) | 29324 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | clear plaques; ranging 3-5 mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 44 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPirate_1 Pirate_2 Pirate_3 Pirate_4 Pirate_5 Pirate_6 Pirate_7 Pirate_8 Pirate_9 Pirate_10 Pirate_11 Pirate_12 Pirate_13 Pirate_14 Pirate_15 Pirate_16 Pirate_17 Pirate_18 Pirate_19 Pirate_20 Pirate_21 Pirate_22 Pirate_23 Pirate_24 Pirate_25 Pirate_26 Pirate_27 Pirate_28 Pirate_29 Pirate_30 Pirate_31 Pirate_32 Pirate_33 Pirate_34 Pirate_35 Pirate_36 Pirate_37 Pirate_38 Pirate_39 Pirate_40 Pirate_41 Pirate_42 Pirate_43 Pirate_44 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR337653 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2012UCLAYM |
| SEA Lysate Titer | 1.8x10^9 pfu/ml |
| Date of SEA Lysate Titering | May 24, 2013 |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | C8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
