| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Judy Odili and Ekaterina Goryunova |
| Year Found | 2017 |
| Location Found | Rosedale, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.359874 N, 76.497645 W Map |
| Discovery Notes | The plaques from the direct plate increased in size and transparency while in the freezer. |
| Naming Notes | We found the sample on a rainy day. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 18, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 57623 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCCGCCT |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI4 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | From our lysogen tests, we could not see any sign of mesa. So we believe Rainydai is likely virulent. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | turbid, sharp margins, visible after 48 hours, transparency increases over a longer period of time |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH155877 |
| Refseq Number | None yet |
| Published in Paper | Blocker D et al 2018, Genome Announcements: Complete Genome Sequences of Six BI Cluster Streptomyces Bacteriophages, HotFries, Moozy, Rainydai, RavenPuff, Scap1, and SenditCS. |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.0 x 10^10 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
