| Detailed Information for Phage RoseMarie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Deborah Jacobs-Sera |
| Year Found | 2024 |
| Location Found | Phalaborwa, South Africa |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 23.78702 S, 31.33633 E Map |
| Discovery Notes | Soil collected while stopped at an Overlook a top a dried river bed |
| Naming Notes | Named after Crystal's first grandbaby! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 20, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1001 |
| Genome length (bp) | 82412 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCTTGCAA |
| GC Content | 61.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Being Annotated (Expected completion by 9/20/2025) |
| Morphotype | Siphoviridae |
| Number of Genes | 141 |
| Number of tRNAs | 18 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |