| Detailed Information for Phage TomBrady |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | TB12 |
| Found By | Chandra Tupurani and Kyle Stoecker |
| Year Found | 2021 |
| Location Found | Richmond, VA United States |
| Finding Institution | Virginia Commonwealth University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.548472 N, 77.45375 W Map |
| Discovery Notes | Found in dirt underneath some mulch. |
| Naming Notes | While we were working on growing isolated plaques for the phage that we found, many struggles were encountered with pipetting. But in the end, we ended up with excellent TEM images. So despite all the struggles, we ended up where we were supposed to, so this phage was named after the comeback kid himself. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3187 |
| Genome length (bp) | 41902 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCCATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Virginia Commonwealth University |
| Annotation Status | In GenBank |
| Plaque Notes | Very clustered, looked like lots of fish eggs in a cluster. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970618 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
145 |
| Pitt Freezer Box Grid# |
E10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
