| Detailed Information for Phage TpudiCK |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Clifford Thupuli |
| Year Found | 2025 |
| Location Found | Cape Town, South Africa |
| Finding Institution | TASK South Africa |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.95551 S, 18.46149 E Map |
| Discovery Notes | The soil sample was taken from the flower bed outside the UCT MCB building. |
| Naming Notes | it is my surname, THUPULI, and my initials, Clifford Kgololosego. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 11, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3394 |
| Genome length (bp) | 80851 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | TASK South Africa |
| Annotation Status | Being Annotated (Expected completion by 2/11/2026) |
| Plaque Notes | The plate had a mixture of both turbid and clear plaque. the turbid plaques were much larger in diameter compared to clear plaque. The 10-4 plate had about 152 plaque, of which less than 50% were turbid, and more than 50% were clear. |
| Number of Genes | 139 |
| Number of tRNAs | 20 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| EM Picture | Download |