| Detailed Information for Phage TymAbreu | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Emma Sweet and Jessica Dahlke |
| Year Found | 2013 |
| Location Found | Hudson, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.984444 N, 92.754444 W Map |
| Discovery Notes | Soil sample was collected at 9:30am on 9-21-13 from a tomato garden approximately 6 inches from a tomato plant and taken from the depth of 30mm. The air temperature at the time of collection was 66 degrees F and the weather was sunny and dry. The soil was amended with compost and was dry and loose. After the enrichment process, we discovered two different sized plaques. Emma chose to isolate the small plaques while Jessica chose to isolate the bigger plaques. After a few isolations processes, the plaques started to vary in size. We then carried through all the experiments and discovered that our phage genomic DNA and electron microscope results were the same. |
| Naming Notes | When Emma and Jessica still thought that they were isolating different phages, Emma chose to name her phage Tyma, a mixture of her and her boyfriend's name, and Jessica chose to name her phage Abreu, after her high school Biology teacher. After discovering the phage was the same, we combined the two names to create TymAbreu. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 267 |
| Genome length (bp) | 15556 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CCCGCGCCACC |
| GC Content | 60.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewArby Arielagos Azathoth Blair CGermain Chestnut Copper Courtney3 Decurro Dewayne Elkhorn Guntur Hunnie Inspire2 Jessica Kels KylieMac Laila Link Lore LouisXIV Maggie Mariposa Massimo Moloch Muttlie Prospero Ronnie Sandman Saphira SerialPhiller Seume Sourignavong StewieGriff Stratus Swenson Taj14 TinoCrisci Toulouse TymAbreu Yank |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear with no halo and range in size from 1mm-2mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 26 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTymAbreu_1 TymAbreu_2 TymAbreu_3 TymAbreu_4 TymAbreu_5 TymAbreu_6 TymAbreu_7 TymAbreu_8 TymAbreu_9 TymAbreu_10 TymAbreu_11 TymAbreu_12 TymAbreu_13 TymAbreu_14 TymAbreu_15 TymAbreu_16 TymAbreu_17 TymAbreu_18 TymAbreu_19 TymAbreu_20 TymAbreu_21 TymAbreu_22 TymAbreu_23 TymAbreu_24 TymAbreu_25 TymAbreu_26 |
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT783672 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.4 x 10^9 |
| Date of SEA Lysate Titering | Oct 15, 2013 |
| Pitt Freezer Box# | 147 |
| Pitt Freezer Box Grid# | H2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
