Mycobacterium phage Zombie
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Detailed Information for Phage Zombie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMonica Towler
Year Found2012
Location FoundLawrenceville, GA USA
Finding InstitutionGeorgia Gwinnett College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.981 N, 84.006 W Map
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 7, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage510
Genome length (bp)41901
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Lysogeny NotesI thought I had multiple phages to begin with because there were multiple different sizes. As it turns out, my phage is Lytic and I kept examining it during a Lytic bursting cycle which made it appear to be many different phages of different size plaques.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaque is clear and approximately 1.5mm in diameter.
MorphotypeSiphoviridae
Number of Genes63
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX664455
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 35
Pitt Freezer Box Grid# 38
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload