| Detailed Information for Phage Nishikigoi |
| Discovery Information |
| Isolation Host | Streptomyces xanthochromogenes NRRL B-5410 |
| Found By | Khoi Tran |
| Year Found | 2016 |
| Location Found | Carrollton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.99963 N, 96.894871 W Map |
| Discovery Notes | Collected from my friend's backyard.
My friend is also my lab partner.
Soil was extracted on September 12, 2016.
Temperature at the time was 20 degrees Celsius
Extracted at 7:14 AM
The site of extraction was next to a concrete slab which is under a palvika plant. The site is also where grease and oil cooking oil would be deposited. The site is a shaded area and was able to stay moist.
The soil extracted was clumped into 3 clumps. It was a dark color and was damp.
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| Naming Notes | Nishikigoi is how carp or koi fish is said in Japanese. This name is homage to my own name as well as my significant other's ethnic background. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 864 |
| Genome length (bp) | 50660 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | CGCCACGTCTT |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BD |
| Subcluster | BD2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Based on tests, Nishikigoi appears to be lysogenic |
| Other Cluster Members |
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| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Round, circular plaques; approximately 2 mm in diameter |
| Number of Genes | 82 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK392364 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^8 pfu/mL |
| Pitt Freezer Box# |
36 |
| Pitt Freezer Box Grid# |
G1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
