Mycobacterium phage PainterBoy
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Detailed Information for Phage PainterBoy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySierra Benner and Hannah Wheatley
Year Found2019
Location FoundTyler, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature4°C
GPS Coordinates32.436683 N, 95.231667 W Map
Discovery NotesWe found our sample from a manure pile in a horse pasture in Tyler, Texas. We retrieved the sample at 9:50 on September 24th. The pile was located under a tree that provides shade throughout most of the day. The area of the pile we took from was moist. The hole created in the pile for sample retrieval was dug where the manure met the ground. The specific pile we took from had a height of approximately 6 inches. We took the sample only from the manure pile, not the ground.
Naming NotesWe choose the name "PainterBoy" because the sample is speculated to have come from the biggest horse in that pasture. That horse's name is Painter Boy. We also saw this name fitting for our phage because the plaques produced are larger than most plaques in our lab.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1695
Genome length (bp)52698
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content61.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA16
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Agaliana (A1)

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Alsfro (A1)

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BPBiebs31 (A1)

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Carlyle (A1)

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ConceptII (A1)

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Ohno789 (A1)

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PacerPaul (A1)

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Parliament (A1)

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PattyP (A1)

Payneful (A1)

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Perseus (A1)

Peterson (A1)

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Chupacabra (A10)

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Insomnia (A11)

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Mabel (A11)

MaCh (A11)

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Munch (A11)

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Petersenfast (A11)

Salz (A11)

Sham4 (A11)

Snape (A11)

Timothy (A11)

TinyTimmy (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Zimmer (A12)

Phlei (A13)

Gail (A14)

Jeeves (A14)

Luchador (A14)

SororFago (A14)

Anaysia (A15)

Anon (A15)

Battleship (A15)

Boohoo (A15)

DekHockey33 (A15)

Epsocamisio (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Looper (A15)

MinecraftSteve (A15)

Nebulosus (A15)

Nedarya (A15)

Oofda (A15)

ReMo (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Switzerland (A15)

Waits (A15)

EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

Baehexic (A2)

BengiVuitton (A2)

Benvolio (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Bugsy (A2)

Caraxes (A2)

Centaur (A2)

Changeling (A2)

Che12 (A2)

ChipMunk (A2)

Colin (A2)

CRB1 (A2)

Crucio (A2)

D29 (A2)

D32 (A2)

Dalmatian (A2)

DBQu4n (A2)

Deloris (A2)

Dignity (A2)

Drake55 (A2)

DudeLittle (A2)

Duplo (A2)

Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

HarryHoudini (A2)

Heffalump (A2)

Herbertwm (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

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L5 (A2)

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Phaded (A2)

Piro94 (A2)

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Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

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Retro23 (A2)

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StarStuff (A2)

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SweetiePie (A2)

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TarsusIV (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

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Updawg (A2)

VA6 (A2)

VC3 (A2)

WeiHuaDA (A2)

Whabigail7 (A2)

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XianYue (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

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AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

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BreSam8 (A3)

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Bxz2 (A3)

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Fernando (A3)

Fred313 (A3)

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GingkoMaracino (A3)

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Grif (A3)

Groupthink (A3)

Grub (A3)

Grum1 (A3)

GtownJaz (A3)

HashRod (A3)

HaveUMetTed (A3)

Heathen (A3)

HelDan (A3)

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Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

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JenCasNa (A3)

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JF2 (A3)

JF4 (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

Lambert1 (A3)

LarryKay (A3)

LBerry (A3)

Lilith (A3)

Louie6 (A3)

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MA5 (A3)

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Manu (A3)

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Microwolf (A3)

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MK4 (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

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P28Green (A3)

Panamaxus (A3)

Pawn (A3)

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Penny1 (A3)

PGHhamlin (A3)

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PhishRPhriends (A3)

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Popcicle (A3)

PotatoSplit (A3)

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Reba (A3)

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Snickers (A3)

SoilDragon (A3)

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SoYo (A3)

Spike509 (A3)

Stagni (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

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Watson (A3)

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AbbysRanger (A4)

Abdiel (A4)

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Alberto7 (A4)

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Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

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Badger (A4)

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Blackmoor (A4)

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Bumblebee11 (A4)

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Camperdownii (A4)

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Eagle (A4)

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Eris (A4)

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Florean (A4)

Flux (A4)

Funston (A4)

Gadost (A4)

HamSlice (A4)

Happiness (A4)

Holli (A4)

Houdini22 (A4)

Iceman (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

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Katalie136 (A4)

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LittleB (A4)

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Lorenzo (A4)

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Mazhar510 (A4)

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Perplexer (A4)

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TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

TroyPia (A4)

TygerBlood (A4)

Ulysses (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

YoSam321 (A4)

AgentM (A5)

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Archetta (A5)

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Bluefalcon (A5)

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Conspiracy (A5)

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ElTiger69 (A5)

ForGetIt (A5)

George (A5)

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HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

Midas2 (A5)

Milcery (A5)

Naca (A5)

PetterN (A5)

Phlorence (A5)

PickleBack (A5)

Scorpia (A5)

Swirley (A5)

SydNat (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

Zolita (A5)

Artemis2UCLA (A6)

BABullseye (A6)

Blinn1 (A6)

Blue7 (A6)

Candra (A6)

Chartreuse (A6)

CloudWang3 (A6)

Cookiedough (A6)

DaVinci (A6)

Dorothea (A6)

EricB (A6)

Garak (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

Roksolana (A6)

SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

ToneTone (A6)

Tucker (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Plaque NotesThe average plaque diameter was 0.58cm on 0.9% top agar.
Has been Phamerated?Yes
Gene List

Click to View

PainterBoy_1

PainterBoy_2
(metalloprotease)

PainterBoy_3

PainterBoy_4
(HNH endonuclease)

PainterBoy_5
(terminase small subunit)

PainterBoy_6

PainterBoy_7
(minor tail protein)

PainterBoy_8

PainterBoy_10

PainterBoy_11
(lysin A)

PainterBoy_12
(holin)

PainterBoy_13
(terminase large subunit)

PainterBoy_14
(portal protein)

PainterBoy_15
(capsid maturation protease)

PainterBoy_16
(scaffolding protein)

PainterBoy_17
(major capsid protein)

PainterBoy_18

PainterBoy_19
(head-to-tail adaptor)

PainterBoy_20

PainterBoy_21
(head-to-tail stopper)

PainterBoy_22

PainterBoy_23
(tail terminator)

PainterBoy_24
(major tail protein)

PainterBoy_25
(tail assembly chaperone)

PainterBoy_26
(tail assembly chaperone)

PainterBoy_27
(tape measure protein)

PainterBoy_28
(minor tail protein)

PainterBoy_29
(minor tail protein)

PainterBoy_30
(minor tail protein)

PainterBoy_31

PainterBoy_32
(minor tail protein)

PainterBoy_33

PainterBoy_34
(Lsr2-like DNA bridging protein)

PainterBoy_35

PainterBoy_36

PainterBoy_37
(ParA-like dsDNA partitioning protein)

PainterBoy_38
(ParB-like dsDNA partitioning protein)

PainterBoy_39

PainterBoy_40

PainterBoy_41

PainterBoy_42

PainterBoy_43

PainterBoy_44

PainterBoy_45
(DNA polymerase I)

PainterBoy_46

PainterBoy_47

PainterBoy_48
(helix-turn-helix DNA-binding domain protein)

PainterBoy_49

PainterBoy_50
(ThyX-like thymidylate synthase)

PainterBoy_51

PainterBoy_52
(ribonucleotide reductase)

PainterBoy_53

PainterBoy_54

PainterBoy_55
(RNA polymerase sigma factor)

PainterBoy_56

PainterBoy_57
(metallophosphoesterase)

PainterBoy_58

PainterBoy_59
(NrdH-like glutaredoxin)

PainterBoy_60

PainterBoy_61
(DNA primase)

PainterBoy_62
(DNA primase)

PainterBoy_63

PainterBoy_64
(endonuclease VII)

PainterBoy_65

PainterBoy_66

PainterBoy_67

PainterBoy_68

PainterBoy_69

PainterBoy_70

PainterBoy_71
(DnaB-like dsDNA helicase)

PainterBoy_72

PainterBoy_73

PainterBoy_74

PainterBoy_75

PainterBoy_76

PainterBoy_77
(Cas4 exonuclease)

PainterBoy_78

PainterBoy_79
(immunity repressor)

PainterBoy_80
(immunity repressor)

PainterBoy_81

PainterBoy_82

PainterBoy_83

PainterBoy_84

PainterBoy_85

PainterBoy_86
(methyltransferase)

PainterBoy_87
(DNA methylase)

PainterBoy_88
(ParB-like nuclease domain protein)

PainterBoy_89

PainterBoy_90

PainterBoy_91

PainterBoy_92

PainterBoy_93

PainterBoy_94

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT639645
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 101
Pitt Freezer Box Grid# F7
Available Files
GenBank File for PhameratorDownload