Mycobacterium phage Acolyte
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Detailed Information for Phage Acolyte
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRobin H. Southwick and Nathan Vonder Haar
Year Found2017
Location FoundWinter Harbor, ME United States of America
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.374232 N, 68.07 W Map
Discovery NotesSample taken from Schoodic Peninsula in Acadia National Park. It was from exposed soil under a tree, next to a cove. Sample taken approximately 7 centimeters from surface.
Naming NotesThe plaques produced from this phage display a prominent "halo".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2007
Genome length (bp)52668
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content63.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA2
Cluster Life CycleTemperate
Other Cluster Members

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Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

BrownWilse (None)

BuddyNugget (None)

BuzzKiehl (None)

Chara (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

LilMac (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

MelBell (None)

MelJoe (None)

MortyC137 (None)

Nakoi (None)

Noodle17 (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Tala (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Arlo (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

Blue (A1)

Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

Brody (A1)

Bruns (A1)

Brutter19 (A1)

Bullseye1 (A1)

Bullseyed (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Cagedmage (A1)

Cedar (A1)

Charlotte (A1)

ConceptII (A1)

Conlectus (A1)

Corky (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

DoodleBob (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

DrFeelGood (A1)

Dulcie (A1)

Dynamix (A1)

EasyLeonard (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Ferocissimum (A1)

FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Francis47 (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

GummyWorm (A1)

HanShotFirst (A1)

HansPay (A1)

HermioneGrange (A1)

Homines (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

JCfoboat (A1)

Kanely (A1)

KatAttack (A1)

KBG (A1)

Kelcifer (A1)

Killigrew (A1)

KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

Long2B (A1)

Lopton (A1)

Magnar (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mimicat (A1)

Minion (A1)

Moose (A1)

Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nepal (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

Osoo (A1)

Oxente (A1)

PacerPaul (A1)

Papez (A1)

Pari (A1)

Parks1 (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

Pinto (A1)

Pippin (A1)

Pita2 (A1)

PSullivan (A1)

Raindrum (A1)

Rhynn (A1)

RidgeCB (A1)

Rigel (A1)

Ringer (A1)

Riporf (A1)

Rocky (A1)

Rohr (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

SargentShorty9 (A1)

Scowl (A1)

Seabiscuit (A1)

SF1Gamma (A1)

SF5Eta (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Snazzy (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

SwissCheese (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Nedarya (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Brooklyn (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kakar (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

Laderlappen (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Malec (A2)

Masha1295 (A2)

Miko (A2)

MissWhite (A2)

MrBarnaby (A2)

Naji (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

NothingSpecial (A2)

Odin (A2)

Ph8s (A2)

Phaded (A2)

Pim618 (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

Tiberius20 (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Bartimeaus (A4)

BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DAgoa1 (A4)

Datway (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

Houdini22 (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nemo27 (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Barbarella (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Pmask (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Cyrano (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Dolasoaandri (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Klaus (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Noelle (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Yecey3 (A9)

Annotation StatusIn GenBank
Plaque NotesExtremely lytic phage that has "haloes" around each plaque (a turbid outer ring).
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Acolyte_1

Acolyte_2
(VIP2-like toxin)

Acolyte_3

Acolyte_4
(HNH endonuclease)

Acolyte_5
(terminase small subunit)

Acolyte_6

Acolyte_8

Acolyte_9
(lysin A)

Acolyte_10
(holin)

Acolyte_11
(lysin B)

Acolyte_12
(terminase large subunit)

Acolyte_13
(portal protein)

Acolyte_14
(capsid maturation protease)

Acolyte_15
(scaffolding protein)

Acolyte_16
(major capsid protein)

Acolyte_17

Acolyte_18
(head-to-tail connector complex protein)

Acolyte_19

Acolyte_20
(head-to-tail connector complex protein)

Acolyte_21

Acolyte_22

Acolyte_23
(major tail protein)

Acolyte_24
(tail assembly chaperone)

Acolyte_25
(tail assembly chaperone)

Acolyte_26
(tape measure protein)

Acolyte_27
(minor tail protein)

Acolyte_28
(minor tail protein)

Acolyte_29
(minor tail protein)

Acolyte_30
(membrane protein)

Acolyte_31
(minor tail protein)

Acolyte_32
(minor tail protein)

Acolyte_33

Acolyte_34
(integrase)

Acolyte_35
(membrane protein)

Acolyte_36

Acolyte_37
(helix-turn-helix DNA binding domain protein)

Acolyte_38

Acolyte_39

Acolyte_40

Acolyte_41
(DNA polymerase I)

Acolyte_42

Acolyte_43
(helix-turn-helix DNA binding domain protein)

Acolyte_44

Acolyte_45
(ThyX-like thymidylate synthase)

Acolyte_46

Acolyte_47
(ribonucleotide reductase)

Acolyte_48

Acolyte_49
(helix-turn-helix DNA binding domain protein)

Acolyte_50

Acolyte_51
(metallophosphoesterase)

Acolyte_52

Acolyte_53
(NrdH-like glutaredoxin)

Acolyte_54
(DNA primase)

Acolyte_55
(DNA primase)

Acolyte_56
(endonuclease VII)

Acolyte_57

Acolyte_58
(esterase)

Acolyte_59

Acolyte_60
(DNA helicase)

Acolyte_61

Acolyte_62
(helix-turn-helix DNA binding domain protein)

Acolyte_63

Acolyte_64
(RecB-like exonuclease/helicase)

Acolyte_65

Acolyte_66
(immunity repressor)

Acolyte_67
(helix-turn-helix DNA binding domain protein)

Acolyte_68
(membrane protein)

Acolyte_69

Acolyte_70

Acolyte_71

Acolyte_72
(membrane protein)

Acolyte_73

Acolyte_74

Acolyte_75

Acolyte_76

Acolyte_77

Acolyte_78

Acolyte_79

Acolyte_80
(membrane protein)

Acolyte_81

Acolyte_82

Acolyte_83

Acolyte_84

Acolyte_85

Acolyte_86

Acolyte_87

Acolyte_88

Acolyte_89

Acolyte_90

Acolyte_91

Acolyte_92

Acolyte_93
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH509441
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 73
Pitt Freezer Box Grid# A1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload