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Mycobacterium phage Phlorence
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Detailed Information for Phage Phlorence
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam PATM
Year Found2012
Location FoundLos Angeles, CA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.065 N, 118.4425 W Map
Discovery NotesDiscovered in Fall of 2012 by Team Phage Against the Machine: Antoine Koehl, Stephanie Yee, Kathleen Tan, and Jonathan Yang.TA: Jovo Vijanderan. Instructor: Payam Benyamini.

Phlorence was isolated from an enrichment culture inoculated with a soil sample taken from under a tree in the UCLA Court of Sciences. The sample was collected on October 3, 2012, by another student Ethan Sim.

DNA preparation was performed by Dr. Krisanavane Reddi (UCLA). Assembly was performed by William Villella (UCLA) and confirmed by Dan Russel (University of Pittsburgh).

Annotation was performed by Team Prophagionals (Marianne Arakelyan, Ivan Guzman, Lilith Jiang, Derek Le) and Edric Yoon. Instructor: Jordan Moberg Parker.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedOct 28, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage92
Genome length (bp)50403
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGGAGGTAA
GC Content60.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA5
Other Cluster Members

Click to View

Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

Brooklyn (None)

BuddyNugget (None)

BuzzKiehl (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

Nakoi (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BigMau (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

Blue (A1)

Bob3 (A1)

BPBiebs31 (A1)

Briton15 (A1)

Brody (A1)

Bruns (A1)

Bullseyed (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Cagedmage (A1)

Cedar (A1)

Charlotte (A1)

ConceptII (A1)

Conlectus (A1)

Corky (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

DoodleBob (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

Dulcie (A1)

Dynamix (A1)

EasyLeonard (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

Ferocissimum (A1)

FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

GummyWorm (A1)

HanShotFirst (A1)

HansPay (A1)

HermioneGrange (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

JCfoboat (A1)

Kanely (A1)

KatAttack (A1)

KBG (A1)

Killigrew (A1)

KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

Long2B (A1)

Lopton (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mimicat (A1)

Minion (A1)

Moose (A1)

Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

Naira (A1)

NEHalo (A1)

Nepal (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

Osoo (A1)

Oxente (A1)

PacerPaul (A1)

Papez (A1)

Pari (A1)

Parks1 (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

Pinto (A1)

Pippin (A1)

Raindrum (A1)

RidgeCB (A1)

Rigel (A1)

Ringer (A1)

Riporf (A1)

Rocky (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

SargentShorty9 (A1)

Scowl (A1)

Seabiscuit (A1)

SF1Gamma (A1)

SF5Eta (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Splat (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Zerstoerer (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Phlei (A13)

Luchador (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshkayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Masha1295 (A2)

Miko (A2)

MissWhite (A2)

MrBarnaby (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

Odin (A2)

Ph8s (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

VirChirotera (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Bartimeaus (A4)

Becaw11 (A4)

BellusTerra (A4)

Blackmoor (A4)

Broseidon (A4)

Bruiser (A4)

Bubbajay (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

Limbeezy3 (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nemo27 (A4)

NorthStar (A4)

Nyxis (A4)

Obama12 (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Suffice (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Whiteangel (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Eidsmoe (A9)

HortumSL17 (A9)

Klaus (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Yecey3 (A9)

Plaque NotesPlaques initially were clear, round circles approximately 0.5 inch in diameter. Subsequent morphology shifted to be more ragged edge, slightly turbid and a 0.25 inch diameter.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes82
Number of tRNAs1
Has been Phamerated?Yes
Gene List

Click to View

Phlorence_1
(HNH endonuclease)

Phlorence_2

Phlorence_3
(Minor tail protein)

Phlorence_4
(minor tail protein)

Phlorence_5

Phlorence_7
(lysin A)

Phlorence_8
(lysin B)

Phlorence_9
(terminase)

Phlorence_10
(portal protein)

Phlorence_11
(capsid maturation protein)

Phlorence_12
(scaffolding protein)

Phlorence_13
(major capsid protein)

Phlorence_14
(head-to-tail connector protein)

Phlorence_15
(head-to-tail connector protein)

Phlorence_16
(head-to-tail connector protein)

Phlorence_17
(head-to-tail connector protein)

Phlorence_18
(head-to-tail connector protein)

Phlorence_19
(major tail protein)

Phlorence_20
(tail assembly chaperone)

Phlorence_21
(tail assembly chaperone)

Phlorence_22
(tape measure protein)

Phlorence_23
(minor tail protein)

Phlorence_24
(minor tail protein)

Phlorence_25
(minor tail protein)

Phlorence_26
(minor tail protein)

Phlorence_27
(minor tail protein)

Phlorence_28
(minor tail protein)

Phlorence_29

Phlorence_30
(integrase, (S-int))

Phlorence_31
(membrane protein)

Phlorence_32
(deoxycytidylate deaminase)

Phlorence_33

Phlorence_34

Phlorence_35

Phlorence_36

Phlorence_37
(DNA polymerase I)

Phlorence_38

Phlorence_39
(helix-turn-helix DNA binding domain)

Phlorence_40
(ThyX thymidylate synthase)

Phlorence_41

Phlorence_42
(ribonucleotide reductase)

Phlorence_43

Phlorence_44

Phlorence_45
(helix-turn-helix DNA binding domain)

Phlorence_46
(phosphoesterase)

Phlorence_47

Phlorence_48

Phlorence_49

Phlorence_50

Phlorence_51
(DNA primase)

Phlorence_52
(DNA primase)

Phlorence_53

Phlorence_54

Phlorence_55

Phlorence_56

Phlorence_57
(endonuclease VII)

Phlorence_58

Phlorence_59

Phlorence_60

Phlorence_61

Phlorence_62

Phlorence_63
(DnaB dsDNA helicase)

Phlorence_64

Phlorence_65

Phlorence_66
(RecB Exonuclease, helicase)

Phlorence_67

Phlorence_68
(immunity repressor)

Phlorence_69
(membrane protein)

Phlorence_70

Phlorence_71

Phlorence_72

Phlorence_73

Phlorence_74

Phlorence_75

Phlorence_76

Phlorence_77

Phlorence_78

Phlorence_79
(antirestriction protein, ArdA-like)

Phlorence_80

Phlorence_81

Phlorence_82

Phlorence_83

Phlorence_84

Phlorence_85

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload