Mycobacterium phage NearlyHeadless
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Detailed Information for Phage NearlyHeadless
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTithe Ahmed
Year Found2015
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.985556 N, 86.455556 W Map
Discovery NotesThe soil sample was obtained at the base of a tree, surrounded by grass on one side and a sidewalk on the other. The immediate surroundings endure moderate foot traffic. The sample was obtained at a temperature of 71°F (at night), roughly 5 cm below the surface. The pH of the soil is unknown.
Naming NotesI named my phage Nearly Headless because after performing electron microscopy, I observed that most of my phages had very weak and/or broken necks. This reminded me of Nearly Headless Nick from Harry Potter, who's head was almost, but not completely, severed from his body.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage286
Sanger Finishing Reactions2
Genome length (bp)52383
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGGATGTAA
GC Content61.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA8
Cluster Life CycleTemperate
Other Cluster Members

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Chargerpower (None)

Abbyshoes (A1)

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EagleEye (A16)

Lucyedi (A16)

PainterBoy (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

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Agape74 (A2)

AN3 (A2)

AN9 (A2)

ANI8 (A2)

AnnaL29 (A2)

Anselm (A2)

ArcherNM (A2)

BabyBack (A2)

Bactobuster (A2)

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D29 (A2)

D32 (A2)

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Drake55 (A2)

DudeLittle (A2)

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Echild (A2)

Equemioh13 (A2)

EvilGenius (A2)

EZMoney23 (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

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TiroTheta9 (A4)

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Wile (A4)

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YoSam321 (A4)

AgentM (A5)

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ElTiger69 (A5)

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LittleCherry (A5)

MarysWell (A5)

Micasa (A5)

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Milcery (A5)

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PetterN (A5)

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PickleBack (A5)

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EricB (A6)

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GreedyLawyer (A6)

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Hammer (A6)

Helmet (A6)

Hexamo (A6)

Hoot (A6)

Indra (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Kipper29 (A6)

Koko (A6)

Lilbunny (A6)

McFly (A6)

Neeharika16 (A6)

Newrala (A6)

Pmask (A6)

Priamo (A6)

Rifter (A6)

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SmellyB (A6)

SuperAwesome (A6)

SuperCallie99 (A6)

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Tucker (A6)

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Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

DroogsArmy (A7)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Danforth (A8)

Dixon (A8)

Expelliarmus (A8)

Groundhog (A8)

NearlyHeadless (A8)

Phillis (A8)

Roary (A8)

Saintus (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Arissanae (A9)

Beemo (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Darrell (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

EmyBug (A9)

ExplosioNervosa (A9)

Fayely (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

SheaKeira (A9)

Spouty (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotating InstitutionWestern Kentucky University
Annotation StatusIn GenBank
Plaque NotesMy plaques are clear and fully form after 48 hours of incubation. After the first 24 hours of incubation, the plaques all seem to be of smaller size. After 48 hours of incubation, some of the plaques mutate to become bigger. The larger plaques have turbid halos.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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NearlyHeadless_1
(HNH endonuclease)

NearlyHeadless_2

NearlyHeadless_3

NearlyHeadless_4

NearlyHeadless_5

NearlyHeadless_7
(lysin A)

NearlyHeadless_8
(lysin B)

NearlyHeadless_9
(terminase large subunit)

NearlyHeadless_10
(portal protein)

NearlyHeadless_11
(capsid maturation protease)

NearlyHeadless_12
(scaffolding protein)

NearlyHeadless_13
(major capsid protein)

NearlyHeadless_14
(head-to-tail adaptor)

NearlyHeadless_15

NearlyHeadless_16
(head-to-tail stopper)

NearlyHeadless_17

NearlyHeadless_18
(tail terminator)

NearlyHeadless_19
(major tail protein)

NearlyHeadless_20
(tail assembly chaperone)

NearlyHeadless_21
(tail assembly chaperone)

NearlyHeadless_22
(tape measure protein)

NearlyHeadless_23
(minor tail protein)

NearlyHeadless_24
(minor tail protein)

NearlyHeadless_25

NearlyHeadless_26

NearlyHeadless_27
(minor tail protein)

NearlyHeadless_28
(minor tail protein)

NearlyHeadless_29

NearlyHeadless_30
(serine integrase)

NearlyHeadless_31

NearlyHeadless_32
(nucleoside deaminase)

NearlyHeadless_33

NearlyHeadless_34

NearlyHeadless_35

NearlyHeadless_36

NearlyHeadless_37

NearlyHeadless_38

NearlyHeadless_39

NearlyHeadless_40

NearlyHeadless_41

NearlyHeadless_42

NearlyHeadless_43
(DNA polymerase I)

NearlyHeadless_44
(helix-turn-helix DNA-binding domain protein)

NearlyHeadless_45

NearlyHeadless_46

NearlyHeadless_47
(ribonucleotide reductase)

NearlyHeadless_48

NearlyHeadless_49

NearlyHeadless_50

NearlyHeadless_51

NearlyHeadless_52
(DNA binding protein)

NearlyHeadless_53
(metallophosphoesterase)

NearlyHeadless_54

NearlyHeadless_55

NearlyHeadless_56

NearlyHeadless_57

NearlyHeadless_58

NearlyHeadless_59
(DNA primase)

NearlyHeadless_60
(DNA primase)

NearlyHeadless_61

NearlyHeadless_62

NearlyHeadless_63

NearlyHeadless_64
(endonuclease VII)

NearlyHeadless_65

NearlyHeadless_66

NearlyHeadless_67

NearlyHeadless_68

NearlyHeadless_69

NearlyHeadless_70

NearlyHeadless_71

NearlyHeadless_72
(helix-turn-helix DNA binding domain protein)

NearlyHeadless_73

NearlyHeadless_74
(DprA-like DNA processing chain A)

NearlyHeadless_75
(phosphoribosyl transferase)

NearlyHeadless_76

NearlyHeadless_77

NearlyHeadless_78
(DnaB-like dsDNA helicase)

NearlyHeadless_79

NearlyHeadless_80

NearlyHeadless_81

NearlyHeadless_82

NearlyHeadless_83
(Cas4 exonuclease)

NearlyHeadless_84

NearlyHeadless_85
(immunity repressor)

NearlyHeadless_86

NearlyHeadless_87

NearlyHeadless_88

NearlyHeadless_89

NearlyHeadless_90

NearlyHeadless_91

NearlyHeadless_92

NearlyHeadless_93

NearlyHeadless_94

NearlyHeadless_95
(ArdA-like antirestriction protein)

NearlyHeadless_96

NearlyHeadless_97

NearlyHeadless_98

NearlyHeadless_99

NearlyHeadless_100

NearlyHeadless_101

NearlyHeadless_102

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH825708
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.02x10^9
Date of SEA Lysate Titering Oct 27, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# F6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload