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BayC (BG) Nesbitt (BL) Azathoth (AN) Copper (AN) Sibs6 (A1)
Mycobacterium phage Gruunaga
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Gruunaga
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNoah Marion Crews
Year Found2015
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.963333 N, 86.368333 W Map
Discovery NotesFound in the backyard of our family home. The phages were taken from soil directly under a compost pile that runs adjacent to a forest and a creek. There was about a cm of wet mud, and underneath was solid, tough earth. A fair medley of muck and good earth were taken.
Naming NotesThe naming of the Phage is based on a popular character in many Role Playing Games. The Barbarian Orc, Gruunaga.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage530
Genome length (bp)52195
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTAA
GC Content61.4%
Sequencing NotesThere is a polymorphism in the population. At position 23564, about 60% of the reads had the "G" that's in the final consensus sequence, while the other 40% had an "A".
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA6
Other Cluster Members

Click to View

Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

Brooklyn (None)

BuddyNugget (None)

BuzzKiehl (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

Nakoi (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

Blue (A1)

Bob3 (A1)

BPBiebs31 (A1)

Briton15 (A1)

Brody (A1)

Bruns (A1)

Bullseyed (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Cagedmage (A1)

Cedar (A1)

Charlotte (A1)

ConceptII (A1)

Conlectus (A1)

Corky (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

DoodleBob (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

Dynamix (A1)

EasyLeonard (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Ferocissimum (A1)

FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

GummyWorm (A1)

HanShotFirst (A1)

HansPay (A1)

HermioneGrange (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

JCfoboat (A1)

Kanely (A1)

KatAttack (A1)

KBG (A1)

Killigrew (A1)

KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

Long2B (A1)

Lopton (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mimicat (A1)

Minion (A1)

Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

NEHalo (A1)

Nepal (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

Osoo (A1)

Oxente (A1)

PacerPaul (A1)

Papez (A1)

Pari (A1)

Parks1 (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PherrisBueller (A1)

PhrostyMug (A1)

Pinto (A1)

Pippin (A1)

RidgeCB (A1)

Rigel (A1)

Ringer (A1)

Riporf (A1)

Rocky (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

SargentShorty9 (A1)

Scowl (A1)

Seabiscuit (A1)

SF1Gamma (A1)

SF5Eta (A1)

Sibs6 (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Splat (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Zerstoerer (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Phlei (A13)

Luchador (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshkayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Masha1295 (A2)

Miko (A2)

MissWhite (A2)

MrBarnaby (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

Odin (A2)

Ph8s (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

VirChirotera (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Bartimeaus (A4)

Becaw11 (A4)

BellusTerra (A4)

Blackmoor (A4)

Broseidon (A4)

Bruiser (A4)

Bubbajay (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

Limbeezy3 (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nemo27 (A4)

NorthStar (A4)

Nyxis (A4)

Obama12 (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Suffice (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Whiteangel (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Eidsmoe (A9)

HortumSL17 (A9)

Klaus (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Yecey3 (A9)

Plaque NotesThe plaque is of the bull's eye variant. Where the center is lysed and the edges of the colony are turbid. It generally needs between a 24-48 hour window to grow at 30 Celsius, and the plaques themselves are large and easy enough to see.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Gruunaga_1

Gruunaga_2

Gruunaga_3

Gruunaga_4
(HNH endonuclease)

Gruunaga_5
(minor tail protein)

Gruunaga_6
(minor tail protein)

Gruunaga_7
(minor tail protein)

Gruunaga_8

Gruunaga_12

Gruunaga_13
(Lysin A)

Gruunaga_14
(Holin)

Gruunaga_15
(Terminase)

Gruunaga_16
(Portal protein)

Gruunaga_17
(Capsid maturation protease)

Gruunaga_18
(Scaffolding protein)

Gruunaga_19
(Major capsid protein)

Gruunaga_20
(head-to-tail connector protein)

Gruunaga_21
(head-to-tail connector protein)

Gruunaga_22
(head-to-tail connector protein)

Gruunaga_23
(head-to-tail connector protein)

Gruunaga_24
(head-to-tail connector protein)

Gruunaga_25
(head-to-tail connector protein)

Gruunaga_26
(Major Tail protein)

Gruunaga_27
(Tail Assembly Chaperone)

Gruunaga_28
(Tail Assembly Chaperone)

Gruunaga_29
(Tape Measure protein)

Gruunaga_30
(Minor Tail Protein)

Gruunaga_31
(Minor Tail protein)

Gruunaga_32
(Minor Tail Protein)

Gruunaga_33
(Minor Tail Protein)

Gruunaga_34
(Minor Tail Protein)

Gruunaga_35

Gruunaga_36

Gruunaga_37
(ParA dsDNA partioning protein)

Gruunaga_38
(ParB dsDNA partiontion protein)

Gruunaga_39

Gruunaga_40
(deoxycytidylate deaminase)

Gruunaga_41

Gruunaga_42

Gruunaga_43

Gruunaga_44

Gruunaga_45
(DNA Polyermase)

Gruunaga_46

Gruunaga_47

Gruunaga_48
(RNA ligase)

Gruunaga_49
(helix-turn-helix DNA binding domain)

Gruunaga_50

Gruunaga_51
(ThyX thymidylate synthase)

Gruunaga_52

Gruunaga_53
(HNH endonuclease)

Gruunaga_54
(Ribonucleotide reductase)

Gruunaga_55

Gruunaga_56

Gruunaga_57

Gruunaga_58
(Recombination Directionality Factor)

Gruunaga_59

Gruunaga_60

Gruunaga_61
(DNA primase)

Gruunaga_62
(DNA Primase)

Gruunaga_63

Gruunaga_64
(Endonuclease VII)

Gruunaga_65

Gruunaga_66

Gruunaga_67

Gruunaga_68

Gruunaga_69

Gruunaga_70

Gruunaga_71
(DnaB dsDNA helicase)

Gruunaga_72

Gruunaga_73

Gruunaga_74

Gruunaga_75
(RecB exonuclease/helicase)

Gruunaga_76

Gruunaga_77
(immunity repressor)

Gruunaga_78

Gruunaga_79

Gruunaga_80

Gruunaga_81

Gruunaga_82

Gruunaga_83

Gruunaga_84

Gruunaga_85
(SprT protease)

Gruunaga_86

Gruunaga_87

Gruunaga_88

Gruunaga_89
(DNA Methylase)

Gruunaga_90
(DNA Methylase)

Gruunaga_91

Gruunaga_92

Gruunaga_93

Gruunaga_94

Gruunaga_95

Gruunaga_96

Gruunaga_97

Gruunaga_98

Gruunaga_99

Gruunaga_100

Gruunaga_101

Gruunaga_102

Gruunaga_103

Gruunaga_104

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX576638
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload