Mycobacterium phage Florean
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Detailed Information for Phage Florean
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElle Humes
Year Found2015
Location FoundBlacksburg, VA United States
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature24°C
GPS Coordinates37.246087 N, 80.425191 W Map
Discovery NotesFlorean was found in an urban area. The ground was flat, between a parking lot and apartment buildings, approximately two meters from trees. The air temperature that day was 24.4 degrees celcius. The soil was slightly damp and the sample was taken from 3cm depth.
Naming NotesFlorean as in Florean Fortescue's Ice Cream Parlor
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4961
Genome length (bp)51371
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTAA
GC Content63.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Other Cluster Members

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Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

Brooklyn (None)

BuddyNugget (None)

BuzzKiehl (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JaBri (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Kimona (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

Nakoi (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

Arcanine (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

Blue (A1)

Bob3 (A1)

BPBiebs31 (A1)

Briton15 (A1)

Brody (A1)

Bruns (A1)

Bullseyed (A1)

Buttons (A1)

Bxb1 (A1)

CactusRose (A1)

Cagedmage (A1)

Cedar (A1)

Charlotte (A1)

ConceptII (A1)

Conlectus (A1)

Corky (A1)

Corvo (A1)

Crispicous1 (A1)

Cueylyss (A1)

DD5 (A1)

DoodleBob (A1)

Doom (A1)

Dreamboat (A1)

DreamCatcher (A1)

Dynamix (A1)

EasyLeonard (A1)

Edtherson (A1)

Euphoria (A1)

Fajezeel (A1)

Fascinus (A1)

Ferocissimum (A1)

FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Froghopper (A1)

Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

GummyWorm (A1)

HanShotFirst (A1)

HansPay (A1)

HermioneGrange (A1)

Hope4ever (A1)

Ichabod (A1)

ILeeKay (A1)

JackSparrow (A1)

Jasper (A1)

JC27 (A1)

JCfoboat (A1)

Kanely (A1)

KatAttack (A1)

KBG (A1)

Killigrew (A1)

KingLeonidas (A1)

KSSJEB (A1)

Kugel (A1)

Kykar (A1)

KyMonks1A (A1)

Lamina13 (A1)

Lesedi (A1)

Littlefoot32 (A1)

Lockley (A1)

Long2B (A1)

Lopton (A1)

Magnito (A1)

Makemake (A1)

Manatee (A1)

Marcell (A1)

Maroc7 (A1)

McGuire (A1)

MetalQZJ (A1)

Michley (A1)

Mimicat (A1)

Minion (A1)

Moter17 (A1)

MPlant7149 (A1)

MrGordo (A1)

Mryolo (A1)

Mule (A1)

Museum (A1)

NEHalo (A1)

Nepal (A1)

Nerujay (A1)

Nhonho (A1)

Niza (A1)

Oogway (A1)

Osoo (A1)

Oxente (A1)

PacerPaul (A1)

Papez (A1)

Pari (A1)

Parks1 (A1)

PattyP (A1)

Pepe (A1)

Perseus (A1)

Petp2012 (A1)

Petruchio (A1)

PhrostyMug (A1)

Pinto (A1)

Pippin (A1)

RidgeCB (A1)

Rigel (A1)

Ringer (A1)

Riporf (A1)

Rocky (A1)

Rufus (A1)

Ruotula (A1)

Rutherferd (A1)

Sagefire (A1)

SarFire (A1)

SargentShorty9 (A1)

Scowl (A1)

Seabiscuit (A1)

SF1Gamma (A1)

SF5Eta (A1)

SkiPole (A1)

Smairt (A1)

Smeagol (A1)

Solon (A1)

SpikeBT (A1)

Spite (A1)

SunTzu (A1)

Switzer (A1)

Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Splat (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Zerstoerer (A10)

Bachome (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Phlei (A13)

Luchador (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

20ES (A2)

AbbyPaige (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshkayV (A2)

Journey13 (A2)

Jsquared (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Masha1295 (A2)

Miko (A2)

MissWhite (A2)

MrBarnaby (A2)

NaSiaTalie (A2)

Nemo (A2)

NicoleTera (A2)

Norris (A2)

Odin (A2)

Ph8s (A2)

Piro94 (A2)

Pomar16 (A2)

Power (A2)

Pukovnik (A2)

QueenB2 (A2)

QueenBeesly (A2)

Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

VirChirotera (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

Assassinator (A3)

AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

Misomonster (A3)

MoneyMay (A3)

MuchMore (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

Pocahontas (A3)

Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

Taurus (A3)

Texage (A3)

Tiffany (A3)

TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

Becaw11 (A4)

BellusTerra (A4)

Blackmoor (A4)

Broseidon (A4)

Bruiser (A4)

Bubbajay (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cindaradix (A4)

Citius (A4)

Clarenza (A4)

Cocoaberry (A4)

Commander (A4)

Connomayer (A4)

DCNugh (A4)

Deano (A4)

Decimator (A4)

Dhanush (A4)

DirtyDunning (A4)

Druantia (A4)

Eagle (A4)

Eapen (A4)

Eris (A4)

Eros (A4)

Flapjack (A4)

Florean (A4)

Flux (A4)

Funston (A4)

FuZGrapes (A4)

Gadost (A4)

Gaius (A4)

HamSlice (A4)

Heisenburg (A4)

Holli (A4)

ICleared (A4)

Iracema64 (A4)

Jaykayelowell (A4)

JetBlade (A4)

JoongJeon (A4)

Kampy (A4)

Katalie136 (A4)

KFPoly (A4)

Kingmustik0402 (A4)

Kratark (A4)

Kyee (A4)

Lemur (A4)

LeoAvram (A4)

LHTSCC (A4)

Limbeezy3 (A4)

LittleB (A4)

LittleGuy (A4)

Lorenzo (A4)

LucyGoose (A4)

Massacre (A4)

Maverick (A4)

Mazhar510 (A4)

Medusa (A4)

MeeZee (A4)

Melvin (A4)

Millski (A4)

Milton (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nemo27 (A4)

NorthStar (A4)

Nyxis (A4)

Obama12 (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Suffice (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Whiteangel (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Benedict (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

Eidsmoe (A9)

HortumSL17 (A9)

Klaus (A9)

Myxus (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Yecey3 (A9)

Plaque NotesThe average size of the plaques was 1.36 mm and the range was 0.55 mm - 2.27 mm. The plaques were fairly round, with clear edges and the area completely clear.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Florean_1
(HNH endonuclease)

Florean_2

Florean_3

Florean_4

Florean_5

Florean_6

Florean_7

Florean_8
(lysin A)

Florean_9
(holin)

Florean_10
(lysin B)

Florean_11
(terminase)

Florean_12
(portal protein)

Florean_13
(capsid maturation protease)

Florean_14
(scaffolding protein)

Florean_15
(major capsid protein)

Florean_16
(head-to-tail connector protein)

Florean_17
(head-to-tail connector protein)

Florean_18
(head-to-tail connector protein)

Florean_19
(head-to-tail connector protein)

Florean_20
(head-to-tail connector protein)

Florean_21
(head-to-tail connector protein)

Florean_22
(major tail protein)

Florean_23
(tail assembly chaperone)

Florean_24
(tail assembly chaperone)

Florean_25
(tape measure protein)

Florean_26
(minor tail protein)

Florean_27
(minor tail protein)

Florean_28
(minor tail protein)

Florean_29
(minor tail protein)

Florean_30
(minor tail protein)

Florean_31
(minor tail protein)

Florean_32

Florean_33
(integrase, (S-int))

Florean_34

Florean_35
(deoxycytidylate deaminase)

Florean_36

Florean_37

Florean_38

Florean_39

Florean_40
(helix-turn-helix DNA binding domain)

Florean_41

Florean_42

Florean_43

Florean_44

Florean_45
(DNA polymerase)

Florean_46
(helix-turn-helix DNA binding protein)

Florean_47

Florean_48
(Thyx thymidylate synthase)

Florean_49

Florean_50
(ribonucleotide reductase)

Florean_51
(HTH DNA binding domain)

Florean_52
(metallophosphoesterase)

Florean_53

Florean_54

Florean_55
(DNA primase)

Florean_56
(DNA Primase)

Florean_57

Florean_58
(endonuclease VII)

Florean_59

Florean_60
(hydrolase)

Florean_61

Florean_62

Florean_63
(phosphoribosyl transferase)

Florean_64
(DnaB dsDNA helicase)

Florean_65

Florean_66

Florean_67

Florean_68
(RecB exonuclease/helicase)

Florean_69

Florean_70
(immunity repressor)

Florean_71

Florean_72

Florean_73

Florean_74

Florean_75

Florean_76

Florean_77

Florean_78
(DNA (cytosine-5) methyltransferase)

Florean_79
(DNA (cytosine-5) methyltransferase)

Florean_80

Florean_81
(SprT protease)

Florean_82

Florean_83

Florean_84

Florean_85

Florean_86

Florean_87

Florean_88

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX557234
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload