Mycobacterium phage Bombshell
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Detailed Information for Phage Bombshell
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Perez and Marisa Trosen
Year Found2016
Location FoundLongview, TX United States of America
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesAt approximately 2:22 PM, soil was gathered into a Ziploc bag with a plastic spoon. The sample was found approximately 1 meter from a short pineleaf tree on a sunny day in and area where it had rained two days prior. In order to gather the sample, a 5.08 centimeter hole was dug. Only about 0.75 g, less than the recommended 1 g, was obtained.
Naming NotesA derivative of the word "serendipity," this phage was named Bombshell because although many issues occurred during the process, we were still able to have relatively large plaques almost immediately and an exceptionally large amount of DNA.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3736
Genome length (bp)51372
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGTAA
GC Content63.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA4
Cluster Life CycleTemperate
Other Cluster Members

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Afua (None)

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Anonia (None)

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Blazer (None)

BlueWhiteSuper (None)

BrownWilse (None)

BuddyNugget (None)

BuzzKiehl (None)

Chara (None)

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Cornelius42 (None)

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Fieldrain (None)

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Norris007 (None)

Ousia (None)

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Blitzkrieg (A10)

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ElSilvo (A10)

Goose (A10)

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Lindberg (A10)

OKCentral2016 (A10)

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Trike (A10)

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WalterMcMickey (A10)

Bachome (A11)

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Bud (A11)

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Et2Brutus (A11)

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Lucivia (A11)

Mabel (A11)

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Munch (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Nedarya (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

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Agape74 (A2)

AnnaL29 (A2)

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ArcherNM (A2)

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Che12 (A2)

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Stumpy (A2)

Superchunk (A2)

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Tiberius20 (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

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Updawg (A2)

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ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

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BabyRay (A3)

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BreSam8 (A3)

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Farber (A3)

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Fred313 (A3)

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Gravata (A3)

Groupthink (A3)

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AgentM (A5)

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LittleCherry (A5)

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Scorpia (A5)

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Tarynearal (A5)

Theia (A5)

Tiger (A5)

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Yolo (A5)

Artemis2UCLA (A6)

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Conquerage (A9)

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Elephantoon (A9)

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Klaus (A9)

LoneWolf (A9)

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PackMan (A9)

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Scherzo (A9)

ShadowHunter (A9)

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Ugenie5 (A9)

Yecey3 (A9)

Annotation StatusOverdue (Expected completion by 5/15/2018)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 58
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
EM PictureDownload