| Detailed Information for Phage BetterYeti |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | E.Ratner, E.James, Z.Farnum, L.Martin |
| Year Found | 2024 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.446884 N, 79.952998 W Map |
| Discovery Notes | Elizabeth A. James, Zora K.Y. Farnum, and Loishooki R. Martin.
Collected off the front lawn of a University of Pittsburgh dormitory.
Dry soil, fine and sandy, sunny, 2pm. |
| Naming Notes | We mutually decided on the name. Yeti was taken, so we had to improvise. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3659 |
| Genome length (bp) | 60438 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Satisfiable bacteria lawn with evident phage activity. We chose our 10E(-6) dilution to proceed further with phage identification and experimentation. |
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915818 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
213 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
