Microbacterium phage Cranjis
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Detailed Information for Phage Cranjis
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByErin Rhoads
Year Found2021
Location FoundUpper Chichester, PA united states of america
Finding InstitutionNeumann University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.856232 N, 75.443149 W Map
Discovery NotesThe sample of which the phage was derived from, was found in a grass field outside of an apartment complex in Upper Chichester, PA. The sample of dirt was dry and not moist.
Naming NotesThe name for this phage is inspired by the show Impractical Jokers. It is short for "Cranjis Mcbasketball", a funny name created by one of the jokers.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 16, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage99
Genome length (bp)53222
Character of genome endsCircularly Permuted
GC Content68.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEC
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNeumann University
Annotation StatusIn GenBank
Plaque NotesPlaques on a plate from late September, 2021 were well isolated, not soo small, but not too big, slightly hazy, and round in shape.
MorphotypeSiphoviridae
Number of Genes91
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Cranjis_1
(terminase small subunit)

Cranjis_2

Cranjis_3
(terminase)

Cranjis_4

Cranjis_5

Cranjis_6

Cranjis_7

Cranjis_8

Cranjis_9

Cranjis_10

Cranjis_11

Cranjis_12

Cranjis_13

Cranjis_14

Cranjis_15

Cranjis_16

Cranjis_17

Cranjis_18

Cranjis_19

Cranjis_20

Cranjis_21
(portal protein)

Cranjis_22

Cranjis_23

Cranjis_24

Cranjis_25

Cranjis_26
(capsid maturation protease)

Cranjis_27

Cranjis_28
(major capsid hexamer protein)

Cranjis_29

Cranjis_30

Cranjis_31
(major tail protein)

Cranjis_32
(head-to-tail adaptor)

Cranjis_33

Cranjis_34

Cranjis_35
(tail assembly chaperone)

Cranjis_36

Cranjis_37
(tape measure protein)

Cranjis_38
(minor tail protein)

Cranjis_39
(minor tail protein)

Cranjis_40
(minor tail protein)

Cranjis_41
(minor tail protein)

Cranjis_42

Cranjis_43

Cranjis_44
(endolysin)

Cranjis_45

Cranjis_46

Cranjis_47

Cranjis_48

Cranjis_49
(Cas4 family exonuclease)

Cranjis_50
(ERF family ssDNA binding protein)

Cranjis_51

Cranjis_52

Cranjis_53
(helix-turn-helix DNA binding domain protein)

Cranjis_54
(ADP-ribosyltransferase)

Cranjis_55
(HNH endonuclease)

Cranjis_56

Cranjis_57

Cranjis_58

Cranjis_59

Cranjis_60
(helix-turn-helix DNA binding domain protein)

Cranjis_61

Cranjis_62

Cranjis_63

Cranjis_64

Cranjis_65
(ssDNA binding protein)

Cranjis_66

Cranjis_67
(helix-turn-helix DNA binding domain protein)

Cranjis_68
(helix-turn-helix DNA binding domain protein)

Cranjis_69

Cranjis_70

Cranjis_71

Cranjis_72

Cranjis_73

Cranjis_74

Cranjis_75

Cranjis_76

Cranjis_77

Cranjis_78

Cranjis_79

Cranjis_80

Cranjis_81

Cranjis_82

Cranjis_83
(DNA binding protein)

Cranjis_84
(ParB-like nuclease domain protein)

Cranjis_85
(glycosyltransferase)

Cranjis_86
(UDP-glucose dehydrogenase)

Cranjis_87
(AAA-ATPase)

Cranjis_88

Cranjis_89

Cranjis_90

Cranjis_91

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP297543
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.49x10^10
Pitt Freezer Box# 135
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload