| Detailed Information for Phage CrunchyBoi |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Cameron Nicklos and Joshua DeKlotz |
| Year Found | 2018 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 47.667222 N, 117.4025 W Map |
| Discovery Notes | Phage found in puddle outside of Hughes Hall at Gonzaga University. Phage enriched and isolated, and grown at room temperature of 25 Degrees Celsius. |
| Naming Notes | Since the phage likes to "eat" bacteria, it was imagined that a "crunchy" sound is made when the bacteria were lysed. Hence the name "CrunchyBoi" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 7, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 270 |
| Genome length (bp) | 53800 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EK |
| Subcluster | EK1 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were consistently clear and circular. Diameter of plaques ranged from 0.2mm-1.0mm. |
| Morphotype | Podoviridae |
| Number of Genes | 57 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW365948 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
94 |
| Pitt Freezer Box Grid# |
B10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
