| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | K. Andersen, O. Donnelly, H. Foree, K. Mathews |
| Year Found | 2022 |
| Location Found | Longview, TX United States of America |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.464444 N, 94.7275 W Map |
| Discovery Notes | The soil sample in which Dulcita was discovered was collected in the bushes behind the Glaske science wing of LeTourneau University in Longview, TX to the right of the dumpsters, fifth bush from the right, in moist, mulchy soil collected on August 23, 2022, at 10:19 a.m. CST. Dulcita was found in a hole that was dug approximately 4cm deep and 8cm wide (elevation was 103 meters). Numerous insects including ants, gnats, and flies were found in the same area. The temperature was 24° C, mostly cloudy and with high humidity. |
| Naming Notes | The name Dulcita was decided upon because it was found at the same time that another phage (which was discovered under a pepper vine) was being isolated. So, that phage was named Koreni (taken from the Czech word for 'spicy') and this phage was named Dulcita (Spanish for 'sweetie'). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 479 |
| Genome length (bp) | 80038 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCCTGCAA |
| GC Content | 61.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | In GenBank |
| Plaque Notes | Dulcita abundantly produced small, clear plaques. They were approximately 1.0 millimeters in diameter with a range of 0.4-1 mm (n = 10). |
| Morphotype | Siphoviridae |
| Number of Genes | 137 |
| Number of tRNAs | 19 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553916 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2x10^10 pfu/ml |
| Pitt Freezer Box# |
150 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
