| Detailed Information for Phage Eddiemania |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Edwin Hernandez-Portillo |
| Year Found | 2023 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | College of Southern Nevada |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.11021 N, 115.2384 W Map |
| Discovery Notes | I decided to walk around the park and look for soil that is dark and moist. Usually next to the trees, the soil was pretty dark and also very humid as well. I collected the sample, did a direct isolation and small phages appeared making it positive. |
| Naming Notes | The name comes from my joy of gaming. I play a lot of video games and decided to add my gamer tag. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 723 |
| Genome length (bp) | 61427 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of Southern Nevada |
| Annotation Status | In GenBank |
| Plaque Notes | the plaques were very small but clustered together. The plaques were round and clear as well. |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184802 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
171 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
