| Detailed Information for Phage Estes |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tess Allen, Alyssa Groscost |
| Year Found | 2017 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 47.665961 N, 117.404007 W Map |
| Discovery Notes | Found near Lake Arthur, beneath a tree in the snow. |
| Naming Notes | It's a street name in Oregon where a friend grew up. Just liked the ring of it. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 808 |
| Genome length (bp) | 83601 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCCCATGCAA |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are cloudy and have blurry margins. They are approximately 2 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 151 |
| Number of tRNAs | 21 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT657341 |
| Refseq Number | None yet |
| Published in Paper | Fitzgerald et al 2021, Microbiology Resource Announcements: Genome Sequences of Subcluster M2 Mycobacteriophages Estes and Aziz |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5x10^8 pfu/mL |
| Date of SEA Lysate Titering |
Mar 8, 2017 |
| Pitt Freezer Box# |
64 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
