Detailed Information for Phage Estes |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Tess Allen, Alyssa Groscost |
Year Found | 2017 |
Location Found | Spokane, WA USA |
Finding Institution | Gonzaga University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 22°C |
GPS Coordinates | 47.665961 N, 117.404007 W Map |
Discovery Notes | Found near Lake Arthur, beneath a tree in the snow. |
Naming Notes | It's a street name in Oregon where a friend grew up. Just liked the ring of it. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 7, 2020 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 808 |
Genome length (bp) | 83601 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 11 bases |
Overhang Sequence | ACCCCATGCAA |
GC Content | 60.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | M |
Subcluster | M2 |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | Gonzaga University |
Annotation Status | In GenBank |
Plaque Notes | Plaques are cloudy and have blurry margins. They are approximately 2 mm in diameter. |
Morphotype | Siphoviridae |
Number of Genes | 151 |
Number of tRNAs | 21 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MT657341 |
Refseq Number | None yet |
Published in Paper | Fitzgerald et al 2021, Microbiology Resource Announcements: Genome Sequences of Subcluster M2 Mycobacteriophages Estes and Aziz |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
5x10^8 pfu/mL |
Date of SEA Lysate Titering |
Mar 8, 2017 |
Pitt Freezer Box# |
64 |
Pitt Freezer Box Grid# |
D12 |
Available Files |
Restriction Digest Picture | Download |
GenBank File for Phamerator | Download |