| Detailed Information for Phage Figliar | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Anna Harshaw |
| Year Found | 2020 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | College of Southern Nevada |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 38°C |
| GPS Coordinates | 36.155333 N, 115.265056 W Map |
| Discovery Notes | Sample picked in a dog park. Fecal matter was present in the area. Relatively moist area due to system of sprinklers. |
| Naming Notes | "Figliar" is an old-fashioned Russian word. It translates as "trickster" or "someone hard to deal with." Due to the lysogenic nature of found bacteriophage, most of the procedures required additional steps to complete. In general, it's complicated to work with. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 793 |
| Genome length (bp) | 61147 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | At first, was thought as a lysogenic phage. Lately revealed to be lytic. Plaques are small in diameter and cloudy. |
| Other Cluster Members |
Click to ViewAflac AlainaMarie Arataki Artorias Avazak BetterYeti BluerMoon Burley Captrips Capybara Chikenjars Citrus Conley Crocheter Dancer Demure Duffington Eddiemania EndAve Fazel Figliar FortCran Gill Gravy Gusicorn Hortihabitatio Hydrus Jodelie19 JulesRay Kenosha Kerry Madvan Magel MajinBuu Mossy Mysticmoon Nadmeg Nithya NoPickles Odesza OhMyWard OlgasClover Perkunas Petito Phepper Pherobrine Rickmore Roney Runhaar Schwartz33 Secretariat ShaggyRogers Talon44 Tanis TenaciousP Trufflozitus Untouchable Vardy Zeph |
| Annotating Institution | College of Southern Nevada |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small in diameter (1mm-2mm), cloudy and hard to see. |
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewFigliar_1 Figliar_2 Figliar_3 Figliar_4 Figliar_5 Figliar_6 Figliar_7 Figliar_8 Figliar_9 Figliar_10 Figliar_11 Figliar_12 Figliar_13 Figliar_14 Figliar_15 Figliar_16 Figliar_17 Figliar_18 Figliar_19 Figliar_20 Figliar_21 Figliar_22 Figliar_23 Figliar_24 Figliar_25 Figliar_26 Figliar_27 Figliar_28 Figliar_29 Figliar_30 Figliar_31 Figliar_32 Figliar_33 Figliar_34 Figliar_35 Figliar_36 Figliar_37 Figliar_38 Figliar_39 Figliar_40 Figliar_41 Figliar_42 Figliar_43 Figliar_44 Figliar_45 Figliar_46 Figliar_47 Figliar_48 Figliar_49 Figliar_50 Figliar_51 Figliar_52 Figliar_53 Figliar_54 Figliar_55 Figliar_56 Figliar_57 Figliar_58 Figliar_59 Figliar_60 Figliar_61 Figliar_62 Figliar_63 Figliar_64 Figliar_65 Figliar_66 Figliar_67 Figliar_68 Figliar_69 Figliar_70 Figliar_71 Figliar_72 Figliar_73 Figliar_74 Figliar_75 Figliar_76 Figliar_77 Figliar_78 Figliar_79 Figliar_80 Figliar_81 Figliar_82 Figliar_83 Figliar_84 Figliar_85 Figliar_86 Figliar_87 Figliar_88 Figliar_89 Figliar_90 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ209301 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.8*10^9pfu/ml |
| Pitt Freezer Box# | 117 |
| Pitt Freezer Box Grid# | E1 |
| Available Files | |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
