| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Anna Harshaw |
| Year Found | 2020 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | College of Southern Nevada |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 38°C |
| GPS Coordinates | 36.155333 N, 115.265056 W Map |
| Discovery Notes | Sample picked in a dog park. Fecal matter was present in the area. Relatively moist area due to system of sprinklers. |
| Naming Notes | "Figliar" is an old-fashioned Russian word. It translates as "trickster" or "someone hard to deal with." Due to the lysogenic nature of found bacteriophage, most of the procedures required additional steps to complete. In general, it's complicated to work with. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 793 |
| Genome length (bp) | 61147 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | At first, was thought as a lysogenic phage. Lately revealed to be lytic. Plaques are small in diameter and cloudy. |
| Other Cluster Members |
|
| Annotating Institution | College of Southern Nevada |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small in diameter (1mm-2mm), cloudy and hard to see. |
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ209301 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.8*10^9pfu/ml |
| Pitt Freezer Box# |
117 |
| Pitt Freezer Box Grid# |
E1 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
