| Detailed Information for Phage FortCran |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Former names | FortNac |
| Found By | Amy LaDue |
| Year Found | 2023 |
| Location Found | Omaha, NE United States |
| Finding Institution | Metropolitan Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 41.223607 N, 95.99068 W Map |
| Discovery Notes | This phage was discovered by Amy LaDue and adopted by Josephine McLean who worked with it from the purification step onwards. It was found from a soil sample collected at 7pm on 10/01/23 under pine trees off the side of the interstate under trash. General description: park, middle of town, Churchich Park. Soil depth was 5-7 inches, it was 88°F, and there was no sun and it was not humid. |
| Naming Notes | This phage is named after the Metro's Fort Omaha campus since my class was the first class to participate in the SEA Phages program at this location. "Cran" is made up of the initials from mine and Amy's pets. C-Cinder (my dog), R-Roxie (Amy's dog), and A and N for Athena and Nala (my beloved rotund cats). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1794 |
| Genome length (bp) | 59848 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Metropolitan Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and vary somewhat in size. |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP978793 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
