| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Jackson Bland, Sydney Miller |
| Year Found | 2020 |
| Location Found | Tampa, FL USA |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 29.511 N, 82.231 W Map |
| Discovery Notes | Tampa Palms 5, collected in compost area near hole #14 under foliage waste; collected at 82F. Soil wet after rain. |
| Naming Notes | The Hydrus constellation essentially means water snake, a type of snake that is nonvenomous to us and swallows its prey alive. Some species are more aggressive than others. We named it Hydrus due to the aquatic aspect, and there was lots of rain prior to the collection of the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 28, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 664 |
| Genome length (bp) | 60842 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic, infected both Gordonia Rubripertincta (host) and Gordonia Terrae. |
| Other Cluster Members |
|
| Annotating Institution | University of South Florida |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were lytic, small/medium sized, mostly clear, some bullseye. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ938589 |
| Refseq Number | None yet |
| Published in Paper | Bland et al 2023, Microbiology Resource Announcements: Annotation of Secretariat and Hydrus, two DJ cluster phages isolated on Gordonia rubripertincta |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.07 x 10^10 PFU/ml |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
