Microbacterium phage Jollipop
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Detailed Information for Phage Jollipop
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByNatalia Martinez
Year Found2023
Location FoundBloomington, IL United States
Finding InstitutionIllinois Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.490008 N, 88.992914 W Map
Discovery NotesConsidering the dirt/grass was recently watered, the area I collected my sample from was slightly muddy. In the specific area that I observed, there were many ants, weeds, and small branches within the dirt. This area was also near a tree so any debris from it may also be within the dirt sample. Some parts of the patch I collected from were also dry and flaky on the top but rich and slightly moldable underneath.
Naming NotesI chose this name for my phage because its named after my favorite stuffed animal that has a lot of emotional significance to me.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 11, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage173
Genome length (bp)53089
Character of genome endsCircularly Permuted
GC Content68.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEC
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesDoesn't seem to form a lysogen.
Other Cluster Members
Annotating InstitutionIllinois Wesleyan University
Annotation StatusIn GenBank
Plaque NotesMy plaque is relatively larger in size. It has cloudy edges and dark inner circles.
Number of Genes90
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Jollipop_1
(terminase small subunit)

Jollipop_2

Jollipop_3
(terminase large subunit)

Jollipop_4

Jollipop_5

Jollipop_6

Jollipop_7

Jollipop_8

Jollipop_9

Jollipop_10

Jollipop_11

Jollipop_12

Jollipop_13

Jollipop_14

Jollipop_15

Jollipop_16

Jollipop_17

Jollipop_18

Jollipop_19

Jollipop_20
(portal protein)

Jollipop_21

Jollipop_22

Jollipop_23
(minor capsid protein)

Jollipop_24

Jollipop_25
(capsid maturation protease)

Jollipop_26

Jollipop_27
(major capsid hexamer protein)

Jollipop_28

Jollipop_29

Jollipop_30
(major tail protein)

Jollipop_31
(head-to-tail adaptor)

Jollipop_32

Jollipop_33

Jollipop_34
(tail assembly chaperone)

Jollipop_35

Jollipop_36
(tape measure protein)

Jollipop_37
(minor tail protein)

Jollipop_38
(minor tail protein)

Jollipop_39
(minor tail protein)

Jollipop_40
(minor tail protein)

Jollipop_41

Jollipop_42

Jollipop_43
(endolysin)

Jollipop_44

Jollipop_45

Jollipop_46

Jollipop_47

Jollipop_48
(exonuclease)

Jollipop_49
(ERF family DNA pairing protein)

Jollipop_50

Jollipop_51

Jollipop_52
(helix-turn-helix DNA binding domain)

Jollipop_53
(ADP-ribosyltransferase)

Jollipop_54
(HNH endonuclease)

Jollipop_55

Jollipop_56

Jollipop_57

Jollipop_58

Jollipop_59

Jollipop_60
(helix-turn-helix DNA binding domain)

Jollipop_61

Jollipop_62
(DNA ligase)

Jollipop_63

Jollipop_64

Jollipop_65
(SSB protein)

Jollipop_66

Jollipop_67
(helix-turn-helix DNA binding domain)

Jollipop_68
(helix-turn-helix DNA binding domain)

Jollipop_69

Jollipop_70

Jollipop_71

Jollipop_72

Jollipop_73

Jollipop_74

Jollipop_75

Jollipop_76

Jollipop_77

Jollipop_78

Jollipop_79

Jollipop_80

Jollipop_81

Jollipop_82

Jollipop_83
(DNA binding protein)

Jollipop_84
(ParB-like nuclease domain)

Jollipop_85
(glycosyltransferase)

Jollipop_86
(UDP-glucose dehydrogenase)

Jollipop_87
(AAA-ATPase)

Jollipop_88

Jollipop_89

Jollipop_90

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978772
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 168
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload