| Detailed Information for Phage Kromp |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Former names | J9DmycrowL |
| Found By | Muhammad Mousa and Benjamin Kelsky |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 38.6409 N, 90.2992 W Map |
| Discovery Notes | Sample collected in Forest Park, from an area with dense moss and rotting grass. Sample was moist, full of organic matter, and possessed a strong scent. Collected at 28 degrees Celsius at a depth of 8 centimeters. |
| Naming Notes | This phage was named for Krombopulos Michael, who deserves to be honored. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 300 |
| Genome length (bp) | 58268 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCGGGGGA |
| GC Content | 71.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | See plaque morphology notes |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques range from <1 to 5 mm in diameter. Often a small number of surviving cells is present within the plaque, these cells exclusively express the gene for red pigment. It is hypothesized (but still unverified) that these cells are lysogenically infected, and that the lysogenic Kromp infection is responsible for this expression. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH744420 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.01*10^11 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
C11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
