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Microbacterium phage FuzzBuster
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Detailed Information for Phage FuzzBuster
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByBrooklynn Herold
Year Found2018
Location FoundCullowhee, NC USA
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.3025 N, 83.183333 W Map
Discovery NotesCreek bed soil from Cullowhee Creek, adjacent to Ramsey center, at the second (older) wooden bridge. Soil was in a well shaded area due to a close by tree, and out of direct exposure from people in consideration of the location on campus.
Naming NotesDue to the fuzzy or cloudy plaques that this specific phage created, I thought it was appropriate to include fuzz into the name. The ending portion of the name was determined by my experience in trying to the phage to grow because it almost disappeared, but I was able to revive the virus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage751
Genome length (bp)54844
Character of genome endsCircularly Permuted
GC Content68.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/30/2019)
Plaque NotesConcistently small specks that vary from clear to cloudy; turbid plaques appear more popular.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^10 PFU/mL
Pitt Freezer Box# 81
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload