Microbacterium phage FuzzBuster
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Detailed Information for Phage FuzzBuster
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByBrooklynn Herold
Year Found2018
Location FoundCullowhee, NC USA
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.3025 N, 83.183333 W Map
Discovery NotesCreek bed soil from Cullowhee Creek, adjacent to Ramsey center, at the second (older) wooden bridge. Soil was in a well shaded area due to a close by tree, and out of direct exposure from people in consideration of the location on campus.
Naming NotesDue to the fuzzy or cloudy plaques that this specific phage created, I thought it was appropriate to include fuzz into the name. The ending portion of the name was determined by my experience in trying to the phage to grow because it almost disappeared, but I was able to revive the virus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage751
Genome length (bp)54844
Character of genome endsCircularly Permuted
GC Content68.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionWestern Carolina University
Annotation StatusIn GenBank
Plaque NotesConcistently small specks that vary from clear to cloudy; turbid plaques appear more popular.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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FuzzBuster_1

FuzzBuster_2
(membrane protein)

FuzzBuster_3

FuzzBuster_4

FuzzBuster_5

FuzzBuster_6
(terminase)

FuzzBuster_7

FuzzBuster_8

FuzzBuster_9

FuzzBuster_10
(membrane protein)

FuzzBuster_11

FuzzBuster_12

FuzzBuster_13

FuzzBuster_14

FuzzBuster_15

FuzzBuster_16

FuzzBuster_17

FuzzBuster_18

FuzzBuster_19

FuzzBuster_20

FuzzBuster_21

FuzzBuster_22

FuzzBuster_23

FuzzBuster_24
(portal protein)

FuzzBuster_25

FuzzBuster_26
(MuF-like minor capsid protein)

FuzzBuster_27
(capsid maturation protease)

FuzzBuster_28

FuzzBuster_29

FuzzBuster_30

FuzzBuster_31

FuzzBuster_32

FuzzBuster_33

FuzzBuster_34

FuzzBuster_35
(head-to-tail adaptor)

FuzzBuster_36

FuzzBuster_37

FuzzBuster_38

FuzzBuster_39

FuzzBuster_40
(tape measure protein)

FuzzBuster_41
(minor tail protein)

FuzzBuster_42
(minor tail protein)

FuzzBuster_43
(minor tail protein)

FuzzBuster_44

FuzzBuster_45

FuzzBuster_46

FuzzBuster_47
(membrane protein)

FuzzBuster_48

FuzzBuster_49
(lysin A)

FuzzBuster_50

FuzzBuster_51

FuzzBuster_52

FuzzBuster_53
(endonuclease VII)

FuzzBuster_54

FuzzBuster_55

FuzzBuster_56

FuzzBuster_57

FuzzBuster_58

FuzzBuster_59

FuzzBuster_60

FuzzBuster_61

FuzzBuster_62

FuzzBuster_63

FuzzBuster_64

FuzzBuster_65

FuzzBuster_66

FuzzBuster_67

FuzzBuster_68

FuzzBuster_69
(methyltransferase)

FuzzBuster_70

FuzzBuster_71

FuzzBuster_72

FuzzBuster_73

FuzzBuster_74
(membrane protein)

FuzzBuster_75

FuzzBuster_76
(DNA helicase)

FuzzBuster_77

FuzzBuster_78
(Ro-like RNA binding protein)

FuzzBuster_79
(exonuclease)

FuzzBuster_80

FuzzBuster_81

FuzzBuster_82

FuzzBuster_83
(DNA primase/polymerase)

FuzzBuster_84

FuzzBuster_85

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN062720
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4x10^10 PFU/mL
Pitt Freezer Box# 81
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload