| Detailed Information for Phage Spartoi | |
| Discovery Information | |
| Isolation Host | Rothia dentocariosa TC5 |
| Former names | Ruth |
| Found By | Trevor Cross |
| Year Found | 2016 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.945132 N, 75.207988 W Map |
| Discovery Notes | This phage was isolated by rinsing the mouth with sterile saline, filtering through a 0.22 um filter, and plating 0.5 mL of filtrate with 0.5 mL stationary bacteria culture onto a Trypsic Soy Agar (TSA) plate with 0.5% Tryptic Soy Top Agar (TSTA) supplemented with 1 mM CaCl2. Plaques formed after 24-48 hours at 37C. |
| Naming Notes | This phage was isolated in an Advanced Microbiology class with several pre-dental majors. In the spirit of dentistry, we pooled everyone's saliva after rinsing with sterile saline in an attempt to find a phage in the oral cavity. To our delight we discovered one phage and also identified the host. We are naming the phage Spartoi, after the mythical people that sprang up from dragon's teeth and are though to be the ancestors of Theban nobility. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 8, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4379 |
| Genome length (bp) | 35189 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCCCCGGCGAT |
| GC Content | 52.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to ViewArV2 Arzan Attoomi Cantare ChewyVIII Chymera DocB7 DS6A E3 EmiRose Eyre Finch Finkle Footloose FuzzBuster GAL1 Gilgamesh GMA1 GMA4 Gudmit Hum25 Ibantik IdentityCrisis Jace Juicebox Kromp Kumao LilSpotty LuckyBarnes Magritte MalagasyRose Min1 MooMoo mu16 OnionKnight OscarSo phiAsp2 phiSAV Pine5 PineapplePizza PSonyx Pumpernickel pZL12 REQ2 REQ3 Reynauld RRH1 Shambre1 Shocker Sleepyhead Sparky Spartoi Success TJE1 TPA2 TPA4 TripleJ Trogglehumper TurkishDelight ValentiniPuff VanLee Whack Wollypog |
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques appearing 1-3 mm across. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSpartoi_1 Spartoi_2 Spartoi_3 Spartoi_4 Spartoi_5 Spartoi_6 Spartoi_7 Spartoi_8 Spartoi_9 Spartoi_10 Spartoi_11 Spartoi_12 Spartoi_13 Spartoi_14 Spartoi_15 Spartoi_16 Spartoi_17 Spartoi_18 Spartoi_19 Spartoi_20 Spartoi_21 Spartoi_22 Spartoi_23 Spartoi_24 Spartoi_25 Spartoi_26 Spartoi_27 Spartoi_28 Spartoi_29 Spartoi_30 Spartoi_31 Spartoi_32 Spartoi_33 Spartoi_34 Spartoi_35 Spartoi_36 Spartoi_37 Spartoi_38 Spartoi_39 Spartoi_40 Spartoi_41 Spartoi_42 Spartoi_43 Spartoi_44 Spartoi_45 Spartoi_46 Spartoi_47 Spartoi_48 Spartoi_49 Spartoi_50 Spartoi_51 Spartoi_52 Spartoi_53 Spartoi_54 Spartoi_55 Spartoi_56 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK061416 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 34 |
| Pitt Freezer Box Grid# | F8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |