Rothia phage Spartoi
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Detailed Information for Phage Spartoi
Discovery Information
Isolation HostRothia dentocariosa TC5
Former namesRuth
Found ByTrevor Cross
Year Found2016
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences (now St. Joseph's Univ)
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates39.945132 N, 75.207988 W Map
Discovery NotesThis phage was isolated by rinsing the mouth with sterile saline, filtering through a 0.22 um filter, and plating 0.5 mL of filtrate with 0.5 mL stationary bacteria culture onto a Trypsic Soy Agar (TSA) plate with 0.5% Tryptic Soy Top Agar (TSTA) supplemented with 1 mM CaCl2. Plaques formed after 24-48 hours at 37C.
Naming NotesThis phage was isolated in an Advanced Microbiology class with several pre-dental majors. In the spirit of dentistry, we pooled everyone's saliva after rinsing with sterile saline in an attempt to find a phage in the oral cavity. To our delight we discovered one phage and also identified the host. We are naming the phage Spartoi, after the mythical people that sprang up from dragon's teeth and are though to be the ancestors of Theban nobility.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 8, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4379
Genome length (bp)35189
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCCCCGGCGAT
GC Content52.4%
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of the Sciences (now St. Joseph's Univ)
Annotation StatusIn GenBank
Plaque NotesClear plaques appearing 1-3 mm across.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK061416
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 34
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload