| Detailed Information for Phage Kumao |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lisa Sako-Glover |
| Year Found | 2015 |
| Location Found | Easton, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | -1°C |
| GPS Coordinates | 40.0 N, 75.0 W Map |
| Discovery Notes | Isolated from wet soil at a depth of 1/2 inch, at the edge of Bushkill Creek in Easton, PA (Forks township). The location of sample collection was under the bridge (north side) of Bushkill Creek Drive at Tatamy Road. The environmental sample was collected on on the morning of January 20, 2015. Despite the winter weather, Bushkill Creek was no frozen and actively flowing. |
| Naming Notes | Named after my father, Kumao Sako, who was a Cancer research physician. He is my inspiration. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 29, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 501 |
| Genome length (bp) | 70373 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCCTCGCGAA |
| GC Content | 62.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | At 24 hours, the plaques were always very small, pinpoint size. At 48 hours plaques were 1mm with an off center halo. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were 1mm in size, clear, with an off center halo that was apparent at 48hours. |
| Morphotype | Siphoviridae |
| Number of Genes | 115 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG009575 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.32 x 10^-13 pfu/ul |
| Pitt Freezer Box# |
15 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
