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Rhodococcus phage Whack
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Detailed Information for Phage Whack
Discovery Information
Isolation HostRhodococcus erythropolis NRRL B-1574
Former namesCamophlage
Found ByEzekiel Robinson, Matthew Cox
Year Found2018
Location FoundOrono, ME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.897511 N, 68.668791 W Map
Discovery NotesTemp: 55 degrees Fahrenheit

Location: University of Maine Orono campus. Soil sample was taken from a decorative garden planted against the side of the green house
Naming NotesWe mistook an agar chunk for our phage, it was camouflaged. Hence, camophlage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage332
Genome length (bp)49660
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTGGCCGCCCTA
GC Content61.9%
Sequencing NotesAround 24100 in this sequence, there were some conflicting reads. There's also a 30-ish bp repeat here. This suggests perhaps there is a sub-population that has a different number (2?) of repeats than the final published sequence (3).
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesSmall circular plaques (.5 mm)
Small circular plaques (1.0 mm)
slightly turbid
Has been Phamerated?Yes
Gene List

Click to View

Whack_1
(terminase, small subunit)

Whack_2
(terminase, large subunit)

Whack_3
(portal protein)

Whack_4
(capsid maturation protease)

Whack_5
(scaffolding protein)

Whack_6
(major capsid protein)

Whack_7

Whack_8
(head-to-tail adaptor)

Whack_9
(head-to-tail stopper)

Whack_10

Whack_11
(tail terminator)

Whack_12
(major tail protein)

Whack_13

Whack_14
(tail assembly chaperone)

Whack_15
(tail assembly chaperone)

Whack_16
(tape measure protein)

Whack_17
(minor tail protein)

Whack_18
(minor tail protein)

Whack_19

Whack_20
(lysin A, protease M23 domain)

Whack_21
(lysin A, glycosyl hydrolase domain)

Whack_22
(holin)

Whack_23

Whack_24

Whack_25
(membrane protein)

Whack_26
(minor tail protein)

Whack_27

Whack_28
(minor tail protein)

Whack_29
(minor tail protein)

Whack_30

Whack_31

Whack_32
(membrane protein)

Whack_33
(helix-turn-helix DNA binding domain)

Whack_34
(membrane protein)

Whack_35

Whack_36
(lipoprotein)

Whack_37
(helix-turn-helix DNA binding domain)

Whack_38
(helix-turn-helix DNA binding domain)

Whack_39
(tyrosine integrase)

Whack_40
(immunity repressor)

Whack_41
(helix-turn-helix DNA binding domain)

Whack_42

Whack_43

Whack_44

Whack_45

Whack_46

Whack_47

Whack_48

Whack_49

Whack_50

Whack_51

Whack_52

Whack_53
(Cas4 family exonuclease)

Whack_54

Whack_55
(ASC-1 transcription coactivator)

Whack_56

Whack_57

Whack_58

Whack_59

Whack_60

Whack_61
(nucleotide pyrophosphohydrolase)

Whack_62

Whack_63

Whack_64
(helix-turn-helix DNA binding domain)

Whack_65

Whack_66

Whack_67
(RuvC-like resolvase)

Whack_68

Whack_69

Whack_70
(helix-turn-helix DNA binding domain)

Whack_71

Whack_72

Whack_73

Whack_74

Whack_75

Whack_76
(HNH endonuclease)

Whack_77
(AAA-ATPase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK967393
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload