Corynebacterium phage Juicebox
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Detailed Information for Phage Juicebox
Discovery Information
Isolation HostCorynebacterium xerosis ATCC 373
Found ByAneesh Pathak and Ote Staton
Year Found2017
Location FoundBirmingham, AL USA
Finding InstitutionUniversity of Alabama at Birmingham
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates33.543682 N, 86.779633 W Map
Discovery NotesSewage influent. The isolation temperature was initially 30 C, but plaques took 72-120 hours to appear. When we moved to the 37 C temperature, the plaques appeared within 48 hours, so we continued to isolate/incubate at 37 C for the sake of time.
Naming NotesThe name comes from the hit movie Kicking and Screaming starring Will Ferrell and NFL Hall of Fame coach Mike Ditka. This movie was influential in our childhoods so we call our phage Juicebox to commemorate our separate but similar pasts.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2017
Genome length (bp)41394
Character of genome endsUnknown
Overhang Length10 bases
Overhang SequenceCGGAGGGCAT
GC Content67.9%
Sequencing Notesat position 3208 bp approximately 25% of reads contain an A instead of consensus G
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of Alabama at Birmingham
Annotation StatusIn GenBank
Plaque NotesThe plaques are consistently small (0.5mm diameters) and cloudy.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH727550
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 59
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload