Mycobacterium phage LilSpotty
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Detailed Information for Phage LilSpotty
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRinah Kim
Year Found2018
Location FoundLa Crescenta, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.223687 N, 118.255645 W Map
Discovery NotesPhage LilSpotty was isolated from moderately moist, dark soil on 4/8/2018 by Rinah Kim at a depth of 4 inches. Air temp at time of collection was 14˚C. The collection site distant from buildings; moderately low foot traffic; approximately 50 m from the major road and cement; no water in close proximity; many trees (more than 15) present within the range of 10 m (the site was located in a local park); slightly sloped.

Phage LilSpotty was isolated by standard enrichment protocol at 35˚C.
High-Titer Lysate [Midi-Lysate] (Archive) Titer: 1.6 * 10^10
Date of High-Titer Lysate Titer: 5/23/18

Characterization was performed by Team We're Goin' Viral (Christian Torres, Kelly Duong, Micah Chong, Chris Lee) and Team KARL (Karina Haleblian, Alexis Ballew, Lillian Lai, Rinah Kim).

MIMG 103AL-S18 Instructor: Dr. Amanda Freise
Teaching Assistant: Samuel Wu
Undergraduate Assistants: Kalin Patel & Mariam Khatchatrian
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 3, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2612
Genome length (bp)49798
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGGCAT
End Determination NotesEnds were fairly apparent from the buildup of reads flanking an overhang, though the right end of the genome had fewer obvious reads supporting the end call.
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterSingleton
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesPlaque Morphology: From the enrichment isolation plates, most of the plaque morphology is tiny and clear but there were some plaques that were turbid.
Average diameter in mm: 2.0 mm
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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LilSpotty_1

LilSpotty_2
(terminase, large subunit)

LilSpotty_3
(portal protein)

LilSpotty_4
(capsid maturation protease)

LilSpotty_5
(scaffolding protein)

LilSpotty_6
(capsid decoration protein)

LilSpotty_7
(major capsid protein)

LilSpotty_8
(head-to-tail adaptor)

LilSpotty_9

LilSpotty_10

LilSpotty_11
(tail terminator)

LilSpotty_12
(major tail protein)

LilSpotty_13
(tail assembly chaperone)

LilSpotty_14
(tail assembly chaperone)

LilSpotty_15
(tape measure protein)

LilSpotty_16
(minor tail protein)

LilSpotty_17

LilSpotty_18
(minor tail protein)

LilSpotty_19
(minor tail protein)

LilSpotty_20
(minor tail protein)

LilSpotty_21
(minor tail protein)

LilSpotty_22
(minor tail protein)

LilSpotty_23
(minor tail protein)

LilSpotty_24

LilSpotty_25
(minor tail protein)

LilSpotty_26

LilSpotty_27

LilSpotty_28

LilSpotty_29

LilSpotty_30

LilSpotty_31
(lysin A)

LilSpotty_32
(lysin B)

LilSpotty_33

LilSpotty_34
(membrane protein)

LilSpotty_35

LilSpotty_36
(membrane protein)

LilSpotty_37
(membrane protein)

LilSpotty_38
(tyrosine integrase)

LilSpotty_39
(toxin in toxin/antitoxin system, RelE-like)

LilSpotty_40
(antitoxin in toxin/antitoxin system, RelB-like)

LilSpotty_41

LilSpotty_42

LilSpotty_43
(immunity repressor)

LilSpotty_44
(helix-turn-helix DNA binding domain)

LilSpotty_45
(G-I-Y Y-I-G endonuclease)

LilSpotty_46
(antirepressor)

LilSpotty_47

LilSpotty_48
(excise)

LilSpotty_49

LilSpotty_50

LilSpotty_51

LilSpotty_52

LilSpotty_53

LilSpotty_54

LilSpotty_55

LilSpotty_56

LilSpotty_57

LilSpotty_58

LilSpotty_59
(WhiB family transcription factor)

LilSpotty_60
(membrane protein)

LilSpotty_61

LilSpotty_62

LilSpotty_63
(RecE-like exonuclease)

LilSpotty_64

LilSpotty_65

LilSpotty_66

LilSpotty_67
(RusA-like resolvase (endonuclease))

LilSpotty_68

LilSpotty_69
(helix-turn-helix DNA binding domain)

LilSpotty_70

LilSpotty_71

LilSpotty_72

LilSpotty_73

LilSpotty_74

LilSpotty_75

LilSpotty_76

LilSpotty_77

LilSpotty_78

LilSpotty_79

LilSpotty_80

LilSpotty_81

LilSpotty_82
(helix-turn-helix DNA binding domain)

LilSpotty_83
(HNH endonuclease)

LilSpotty_84

LilSpotty_85
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK977707
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 78
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload