| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Aaron Cristan, Phillip Gonzales |
| Year Found | 2015 |
| Location Found | Corpus Christi, TX USA |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.750184, -97.375730 Map |
| Discovery Notes | -Discovered in a moist bank of a canal leading into a creek.
-Removed vegetation to expose soft soil, with green moss overgrown on the surface.
-Runoff from canal comes from storm drain systems within residential area.
-Canal leads out into OSO Creek with high vegetation and then runs out into Corpus Christi bay.
-Construction 25m from original isolation site.
-Weather conditions: Partly Cloudy, Breezy, est. Temp 95F
-Water conditions: Green, Slow current and Visibility 1/2ft to 1ft .
-Wildlife: Small schools of mullet, mosquitos, and common insects found around river banks in S. Texas. |
| Naming Notes | -Nanosmite's name derives from two meanings.
-Nano is to described the unit of measurement to determine the Phage's size.
Smite- meaning to destroy, was named bases on the a highly active lytic cycle. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 470 |
| Genome length (bp) | 82676 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | ACCTCTTGCAA |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | M |
| Subcluster | M3 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | No lysogeny evident only lytic cycle present |
| Other Cluster Members |
|
| Annotating Institution | Carthage College |
| Annotation Status | In GenBank |
| Plaque Notes | -created tiny clear plaques at astonishing rate.
-radius of plaque is .5 mm
-small and quick forming plagues |
| Morphotype | Siphoviridae |
| Number of Genes | 150 |
| Number of tRNAs | 20 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW578836 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0E10 |
| Date of SEA Lysate Titering |
Oct 12, 2015 |
| Pitt Freezer Box# |
24 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
