| Detailed Information for Phage Nithya | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Xavier Rodriguez and Haley Sawyer |
| Year Found | 2020 |
| Location Found | Lincoln, NE United States of America |
| Finding Institution | University of Nebraska-Lincoln |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 40.8195 N, 96.6845 W Map |
| Discovery Notes | The soil sample came from a community garden. The depth of soil was around 2cm and the soil was also dry and dusty. The garden was jointly Nithya’s and Ellie’s. |
| Naming Notes | Found by Xavier. Xavier: I called my friend last minute asking if I could take her soil, so it's only fair that I name the phage after her. I would like to thank Ellie for letting me come to her garden on such short notice. I also want to thank Haley for being such a great lab partner this semester (F2020) and Dr. Chia for teaching this course. Pronounced: Nih-thyah like “with ya”. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 374 |
| Genome length (bp) | 61092 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCTCT |
| GC Content | 51.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Because the plaques are so small, it is hard to tell whether Nithya is lytic or lysogenic. We believe that Nithya is lytic because the plaques tend to be clear and not turbid. |
| Other Cluster Members |
Click to ViewAflac AlainaMarie Arataki Artorias Avazak BluerMoon Burley Captrips Capybara Chikenjars Conley Crocheter Duffington Eddiemania EndAve Fazel Figliar FortCran Gill Gravy Gusicorn Hortihabitatio Hydrus Jodelie19 JulesRay Kenosha Kerry Madvan Magel Mossy Mysticmoon Nadmeg Nithya NoPickles Odesza OhMyWard OlgasClover Perkunas Petito Phepper Pherobrine Rickmore Roney Runhaar Schwartz33 Secretariat ShaggyRogers Tanis TenaciousP Untouchable Vardy Zeph |
| Annotating Institution | University of Nebraska-Lincoln |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are very small (less than 0.5mm on average). The plaques do not appear to be turbid and they have very clear edges. Average capsid diameter: 69nm Average tail length: 280nm |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewNithya_1 Nithya_2 Nithya_3 Nithya_4 Nithya_5 Nithya_6 Nithya_7 Nithya_8 Nithya_9 Nithya_10 Nithya_11 Nithya_12 Nithya_13 Nithya_14 Nithya_15 Nithya_16 Nithya_17 Nithya_18 Nithya_19 Nithya_20 Nithya_21 Nithya_22 Nithya_23 Nithya_24 Nithya_25 Nithya_26 Nithya_27 Nithya_28 Nithya_29 Nithya_30 Nithya_31 Nithya_32 Nithya_33 Nithya_34 Nithya_35 Nithya_36 Nithya_37 Nithya_38 Nithya_39 Nithya_40 Nithya_41 Nithya_42 Nithya_43 Nithya_44 Nithya_45 Nithya_46 Nithya_47 Nithya_48 Nithya_49 Nithya_50 Nithya_51 Nithya_52 Nithya_53 Nithya_54 Nithya_55 Nithya_56 Nithya_57 Nithya_58 Nithya_59 Nithya_60 Nithya_61 Nithya_62 Nithya_63 Nithya_64 Nithya_65 Nithya_66 Nithya_67 Nithya_68 Nithya_69 Nithya_70 Nithya_71 Nithya_72 Nithya_73 Nithya_74 Nithya_75 Nithya_76 Nithya_77 Nithya_78 Nithya_79 Nithya_80 Nithya_81 Nithya_82 Nithya_83 Nithya_84 Nithya_85 Nithya_86 Nithya_87 Nithya_88 Nithya_89 Nithya_90 Nithya_91 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ388556 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 121 |
| Pitt Freezer Box Grid# | A7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |