| Detailed Information for Phage Pherobrine |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Abigail Leonard and Cynthia Reagan |
| Year Found | 2021 |
| Location Found | Durham, NC United States |
| Finding Institution | Durham Technical Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 25°C |
| GPS Coordinates | 36.118889 N, 78.864722 W Map |
| Discovery Notes | Found outside of the pool I work at. It was the day after a storm so the ground was still a little soggy. The sample was found under a bush in a shaded area. |
| Naming Notes | We named our phage after the Minecraft antihero, herobrine |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 12, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1543 |
| Genome length (bp) | 60305 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 51.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DJ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Durham Technical Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Average plaque size: ~1.5mm
Average Phage Size: ~375 nm
Uniform or Un-uniform: Uniform
Turbid or Clear: Clear |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970572 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.24 x 10 ^9 pfu/ml |
| Pitt Freezer Box# |
142 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
