The Actinobacteriophage Database

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Detailed Information for Phage Phreak
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Petros Raygoza
Year Found	2012
Location Found	Spokane, WA USA
Finding Institution	Gonzaga University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	37°C
GPS Coordinates	47.66709, -117.40304 Map
Discovery Notes	from a flowerbed, sunny and 80 degrees Fahrenheit
Naming Notes	Named after SF Giants pitcher Tim Lincecum, "The Freak".
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 23, 2014
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454
Approximate Shotgun Coverage	460
Sanger Finishing Reactions	8
Genome length (bp)	41901
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CCCCATGGCAT
GC Content	66.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	G
Subcluster	G1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Angel (G1) Annihilator (G1) Aroostook (G1) Avrafan (G1) AzulaCat (G1) Barkley26 (G1) BPs (G1) BQuat (G1) BruceB (G1) Camri (G1) CassieYates (G1) Cedasite (G1) Chance64 (G1) Cherrybomb426 (G1) CLED96 (G1) Coleslaw (G1) Crespo (G1) Darionha (G1) DMoney (G1) ECartman (G1) Frosty24 (G1) Gideon (G1) GoldenAsh (G1) Gomashi (G1) Grizzly (G1) Halo (G1) Hope (G1) Hotshotbaby7 (G1) Jane (G1) Jolene (G1) Jonghyun (G1) JorRay (G1) Kareem (G1) Kasen3 (G1) Liefie (G1) LouisV14 (G1) Maliketh (G1) Marmie (G1) Mowgli (G1) OctaviousRex (G1) Olga (G1) Paito (G1) Peeb (G1) Periodt (G1) Phish (G1) Phreak (G1) PinkYoshi (G1) Plagueis (G1) Rabbs (G1) Remy19 (G1) Renaissance (G1) Schiebel (G1) ShaboiShabazz (G1) Sizemore (G1) Sneeze (G1) Sweets (G1) Taheera (G1) Terror (G1) TomBrady (G1) Wendigo (G1) ZoMa (G1) Zombie (G1) Avocado (G2) Cambiare (G2) FlagStaff (G2) Pace1224 (G2) MOOREtheMARYer (G3) Pinnie (G3) Jolie2 (G4) Lemuria (G4) Mercurio (G4) Antsirabe (G5) Stargaze (G5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Morphotype	Siphoviridae
Number of Genes	63
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Phreak_1 Phreak_2 (terminase) Phreak_3 (portal protein) Phreak_4 (capsid maturation protease) Phreak_5 Phreak_6 (scaffolding protein) Phreak_7 (major capsid protein) Phreak_8 (head-to-tail connector) Phreak_9 (head-to-tail adaptor) Phreak_10 (head-to-tail stopper) Phreak_11 Phreak_12 (tail terminator) Phreak_13 (major tail protein) Phreak_14 (tail assembly chaperone) Phreak_15 (tail assembly chaperone) Phreak_16 (tape measure protein) Phreak_17 (minor tail protein) Phreak_18 (minor tail protein) Phreak_19 (minor tail protein) Phreak_20 (minor tail protein) Phreak_21 (minor tail protein) Phreak_22 (minor tail protein) Phreak_23 Phreak_24 Phreak_25 Phreak_26 Phreak_27 (lysin A) Phreak_28 (lysin B) Phreak_29 (holin) Phreak_30 Phreak_31 Phreak_32 (integrase) Phreak_33 (immunity repressor) Phreak_34 (recombination directionality factor) Phreak_35 Phreak_36 Phreak_37 Phreak_38 Phreak_39 Phreak_40 Phreak_41 Phreak_42 (exonuclease) Phreak_43 (ssDNA binding protein) Phreak_44 Phreak_45 Phreak_46 Phreak_47 Phreak_48 Phreak_49 Phreak_50 Phreak_51 (RuvC-like resolvase) Phreak_52 Phreak_53 (MPME1 protein) Phreak_54 Phreak_55 Phreak_56 Phreak_57 Phreak_58 Phreak_59 Phreak_60 Phreak_61 Phreak_62 (HNH endonuclease) Phreak_63
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KT347314
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	36
Pitt Freezer Box Grid#	8
Available Files
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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