| Detailed Information for Phage Sneeze |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Marina Grossi |
| Year Found | 2014 |
| Location Found | Palo Verde, Costa Rica |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 10.4 N, 85.32 W Map |
| Discovery Notes | I "adopted" Sneeze from Joseph Skibbens, a member of my SEA Phages course. Since I consistently did not get plaques on any of my plates, I obtained a plaque from one of Joseph's plates that had two plaques with very different morphologies. At the time the soil was collected in early January, the temperature was 85 degrees Fahrenheit and it was taken at a depth of 0.5". It was collected from a recently dried-up marsh. |
| Naming Notes | I named Sneeze after the nickname my family gave to my dog. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 10, 2014 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent |
| Approximate Shotgun Coverage | 3162 |
| Genome length (bp) | 42429 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCCCATGGCAT |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | G |
| Subcluster | G1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are usually 1-2 mm in diameter, with turbid (but not very cloudy) centers. These plaques are surrounded by a very turbid "halo" that can easily be seen when held up to a light source. When left in the incubator, these "halos" can grow in diameter (about 1 mm after the 72 hour test). |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX534004 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.00x10^10 pfu/ml |
| Date of SEA Lysate Titering |
Mar 12, 2014 |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
