Microbacterium phage Wesak
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| Detailed Information for Phage Wesak | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Hailey Kerns |
| Year Found | 2018 |
| Location Found | Spring Hill, FL USA |
| Finding Institution | Saint Leo University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 24°C |
| GPS Coordinates | 28.329722 N, 82.475278 W Map |
| Discovery Notes | Sample #1 was taken at 7:48 am. The soil sample taken was moist and a little clumped with a few pieces of grass and root in it. The sample was taken from about 8 inches deep underneath the grass. The grass in the area was dewy and healthy. The sample was taken near the houses’ septic system (approximately 3 ft below the surface) which is in our dog yard where 8 golden retrievers defecate and urinate frequently. |
| Naming Notes | I chose the name Wesak because of my Asian water monitor that recently passed away. She is incredibly important to me and I miss her dearly. Her name itself represents the vitality of humanity’s aspirations. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 419 |
| Genome length (bp) | 53789 |
| Character of genome ends | Circularly Permuted |
| GC Content | 56.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EK |
| Subcluster | EK1 |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Saint Leo University |
| Annotation Status | In GenBank |
| Plaque Notes | 3mm in size; clear and irregular edges; slightly cloudy plaque |
| Morphotype | Podoviridae |
| Number of Genes | 54 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN369747 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 74 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| EM Picture | Download |
| GenBank File for Phamerator | Download |